alphaB-crystallin is phosphorylated during myocardial infarction: involvement of platelet-derived growth factor-BB.

Abstract

alphaB-crystallin is the most abundant low-molecular-weight heat shock protein in heart and recent studies have demonstrated that it plays a cardioprotective role during myocardial infarction both in vivo and in vitro. On the other hand, platelet-derived growth factor (PDGF), a potent serum mitogen, has been reported to improve cardiac function after myocardial infarction. In the present study, using a mouse myocardial infarction model, we investigated whether alphaB-crystallin is phosphorylated during myocardial infarction and the implication of PDGF-BB. Phosphorylation of alphaB-crystallin at Ser-59 was time dependently induced and plasma PDGF-BB levels were concomitantly increased. Moreover, PDGF-BB-stimulated phosphorylation of alphaB-crystallin was suppressed by SB203580, a specific inhibitor of p38 mitogen-activated protein (MAP) kinase, in primary cultured cardiac myocytes. Our results indicate that PDGF-BB induces phosphorylation of alphaB-crystallin via p38 MAP kinase during myocardial infarction.

• Authors: En Shu, Hiroyuki Matsuno, Shigeru Akamastu, Yosuke Kanno, Hidetaka Suga, Keiichi Nakajima, Akira Ishisaki, Shinji Takai, Kanefusa Kato, Yasuo Kitajima, Osamu Kozawa
• Journal: Archives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 438 Issue 2 Pg. 111-8 (Jun 15 2005) ISSN: 0003-9861 [Print] United States
• PMID: 15907784 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Flavonoids
• Imidazoles
• Platelet-Derived Growth Factor
• Proto-Oncogene Proteins c-sis
• Pyridines
• alpha-Crystallin B Chain
• Becaplermin
• Serine
• p38 Mitogen-Activated Protein Kinases
• SB 203580
• 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

Topics

• Animals
• Becaplermin
• Blotting, Western
• Disease Models, Animal
• Enzyme Inhibitors (pharmacology)
• Flavonoids (pharmacology)
• Gene Expression Regulation
• Heart Ventricles (pathology)
• Imidazoles (pharmacology)
• Immunohistochemistry
• MAP Kinase Signaling System
• Male
• Mice
• Mice, Inbred C57BL
• Myocardial Infarction (metabolism)
• Myocardium (pathology)
• Phosphorylation
• Platelet-Derived Growth Factor (metabolism)
• Proto-Oncogene Proteins c-sis
• Pyridines (pharmacology)
• Serine (metabolism)
• Time Factors
• alpha-Crystallin B Chain (metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)