A novel DNA vaccine containing four mimicry epitopes for gastric cancer.
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| Abstract |
Gastric cancer is one of the most common malignant tumors in China. This paper focuses on the development of a DNA vaccine containing four mimotopes of MG7Ag for gastric cancer (multi-epitope vaccine). By inoculating BALB/c mice, the vaccine was characterized and compared with a similar vaccine containing only one mimotope (mono-epitope vaccine) and other controls. Cellular ELISA indicated that serum titer of antibody against MG7Ag was significantly higher in mice immunized with the multi-epitope vaccine than that in the group immunized with the mono-epitope vaccine (0.8627 vs 0.6754, P < 0.05). And ELISPOT assay showed that the number of INF-gamma spots induced by multi-epitope vaccine was significantly larger than that of the group immunized with mono-epitope vaccine(93.3 vs 70.7, P < 0.05). Two weeks after tumor challenge, the weight of tumor in each mouse was evaluated, and the tumor masses formed in the mice immunized with multi-epitope vaccine were markedly smaller than those formed in the mice immunized with mono-epitope vaccine. These studies demonstrated that both humoral and cellular response were induced by the two vaccines and the efficiency of multi-epitope vaccine is stronger than that of the mono-epitope vaccine.
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| Authors | Yu Chen, Kaichun Wu, Changcun Guo, Changjiang Liu, Shuang Han, Tao Lin, Xiaoxuan Ning, Rui Shi, Yongquan Shi, Daiming Fan |
| Journal | Cancer biology & therapy (Cancer Biol Ther) Vol. 4 Issue 3 Pg. 308-12 (Mar 2005) ISSN: 1538-4047 [Print] United States |
| PMID | 15876863 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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