Abstract | PURPOSE: EXPERIMENTAL DESIGN: CD4(+) or CD4(+)CD14(-) cells were purified from peripheral blood mononuclear cells of 11 ATL patients with cutaneous involvement and normal healthy volunteers. Tissue-infiltrating cells were isolated from skin tumors. The expression of chemokine receptors on these cells were analyzed by flow cytometry. The production of chemokines and cytokines by the neoplastic cells was assessed by ELISA and reverse transcription-PCR after cultivation for 96 hours in the presence or absence of anti-CD3/CD28 monoclonal antibodies. Finally, TARC and CCR4 expressions were examined by immunohistochemistry. RESULTS: ATL cells highly expressed CCR4 but did not necessarily exhibit the Th2 cytokine profile. The cells also produced TARC and MDC. The production level of MDC was higher in the skin tumor formation group than that in the nontumor group. Immunohistochemically, both CCR4 and TARC were expressed by the tumor cells in the lesional skin. CONCLUSIONS: ATL cells not only express CCR4 but also produce TARC and MDC. The skin tumor formation as well as the monoclonal integration of proviral DNA are the factors that are associated with the high production of Th2 chemokines by ATL cells.
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Authors | Takatoshi Shimauchi, Satoshi Imai, Ryosuke Hino, Yoshiki Tokura |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 11
Issue 6
Pg. 2427-35
(Mar 15 2005)
ISSN: 1078-0432 [Print] United States |
PMID | 15788694
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- CCL22 protein, human
- CCR4 protein, human
- Chemokine CCL22
- Chemokines, CC
- Cytokines
- Ligands
- Receptors, CCR4
- Receptors, Chemokine
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Antibodies, Monoclonal
- Binding Sites, Antibody
- CD4-Positive T-Lymphocytes
(metabolism)
- Chemokine CCL22
- Chemokines, CC
(immunology, metabolism)
- Cytokines
(immunology, metabolism)
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Leukemia-Lymphoma, Adult T-Cell
(genetics, immunology, metabolism)
- Ligands
- Male
- Middle Aged
- Receptors, CCR4
- Receptors, Chemokine
(genetics, immunology, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Skin Neoplasms
(genetics, immunology, metabolism)
- Th2 Cells
(cytology, immunology, metabolism)
- Thymus Gland
(immunology, metabolism)
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