Mucosal targeting of allergen-loaded microspheres by Aleuria aurantia lectin.

Abstract

Murine intestinal M-cells express alpha-L-fucose residues. We constructed alpha-L-fucose-targeting particles for oral immunotherapy of IgE-mediated allergy. Poly(D,L-lactic-co-glycolic acid)-microspheres were loaded with birch pollen allergens, and functionalised with the alpha-L-fucose specific Aleuria aurantia lectin (AAL). The AAL-microspheres had a size of 1-3 microm, protected the entrapped allergens from gastric degradation and released 46.6+/-1.3% allergen over 21 days in vitro. Oral gavages of AAL-particles to naive BALB/c mice induced birch pollen-specific IgG2a, but not IgG1 antibodies. We conclude that targeting allergens to alpha-L-fucose-receptor bearing cells using AAL-microspheres induces specific Th1-antibody responses possibly counteracting Th2-dominated allergy, and therefore provides a potentially useful formulation for oral immunotherapy.

• Authors: Franziska Roth-Walter, Isabella Schöll, Eva Untersmayr, Adolf Ellinger, George Boltz-Nitulescu, Otto Scheiner, Franz Gabor, Erika Jensen-Jarolim
• Journal: Vaccine (Vaccine) Vol. 23 Issue 21 Pg. 2703-10 (Apr 15 2005) ISSN: 0264-410X [Print] Netherlands
• PMID: 15780716 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Immunoglobulin G
• Lectins
• Polymers
• lectin, Aleuria aurantia
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polyglycolic Acid
• Lactic Acid

Topics

• Allergens (administration & dosage)
• Animals
• Betula (immunology)
• Caco-2 Cells
• Desensitization, Immunologic
• Enterocytes (metabolism)
• Female
• Humans
• Immunoglobulin G (blood)
• Lactic Acid (administration & dosage)
• Lectins (administration & dosage, metabolism)
• Mice
• Mice, Inbred BALB C
• Microspheres
• Pollen (immunology)
• Polyglycolic Acid (administration & dosage)
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polymers (administration & dosage)