[Role of mitogen - activated protein kinases signal pathway in cardiomyocyte injury induced by serum after hypoxia and burn injury].

Abstract

OBJECTIVE:

To observe the activation and explore the role of three major mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated kinase (ERK), p38 kinase and c-Jun NH(2)-terminal protein kinase (JNK), in cardiomyocytes injury induced by serum after hypoxia and burn injury.

METHODS:

Phosphorylation of the three major MAPKs in primary cultured neonatal rat cardiomyocytes were determined by Western blotting. Contents of released lactate dehydrogenases (LDH) and death-rate of myocytes treated with serum after hypoxia and burn injury, SB203580+hypoxia and burn serum, PD98059+hypoxia and burn serum were observed respectively.

RESULTS:

Exposing rat neonatal cardiomyocytes to hypoxia and burn serum resulted in a rapid and prolonged activation of p38 kinase and ERK. Phosphorylation degree of p38 kinase, ERK1/2 was increased. Myocytes treated with SB203580 (10 micromol/L), a selective inhibitor of p38 kinase, resulted in a significant decline in LDH leakage leaking and cell death. However, with pretreatment of cell with PD98059(25 micromol/L), an inhibitor of ERK, LDH leakage and cell death were increased.

CONCLUSION:

Serum obtained after hypoxia and burn injury activate p38 kinase and ERK, but not JNK, in cardiomyocytes. p38 kinase pathway might play a role in mediating cardiomyocytes injury, whereas ERK plays a protective role.

• Authors: Jia-ping Zhang, Yue-sheng Huang, Zong-cheng Yang
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 17 Issue 3 Pg. 150-3 (Mar 2005) ISSN: 1003-0603 [Print] China
• PMID: 15760524 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Extracellular Signal-Regulated MAP Kinases
• JNK Mitogen-Activated Protein Kinases
• Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Burns (blood)
• Cells, Cultured
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Hypoxia (blood)
• JNK Mitogen-Activated Protein Kinases (metabolism)
• MAP Kinase Signaling System (physiology)
• Mitogen-Activated Protein Kinases (metabolism)
• Myocytes, Cardiac (enzymology, pathology)
• Phosphorylation
• Rats
• Serum
• p38 Mitogen-Activated Protein Kinases (metabolism)