Abstract |
Pheromones are cell type-specific signals used for communication between individuals of the same species. When faced with overcrowding or starvation, Caenorhabditis elegans secrete the pheromone daumone, which facilitates communication between individuals for adaptation to adverse environmental stimuli. Daumone signals C. elegans to enter the dauer stage, an enduring and non-ageing stage of the nematode life cycle with distinctive adaptive features and extended life. Because daumone is a key regulator of chemosensory processes in development and ageing, the chemical identification of daumone is important for elucidating features of the daumone-mediated signalling pathway. Here we report the isolation of natural daumone from C. elegans by large-scale purification, as well as the total chemical synthesis of daumone. We present the stereospecific chemical structure of purified daumone, a fatty acid derivative. We demonstrate that both natural and chemically synthesized daumones equally induce dauer larva formation in C. elegans (N2 strain) and certain dauer mutants, and also result in competition between food and daumone. These results should help to elucidate the daumone-mediated signalling pathway, which might in turn influence ageing and obesity research and the development of antinematodal drugs.
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Authors | Pan-Young Jeong, Mankil Jung, Yong-Hyeon Yim, Heekyeong Kim, Moonsoo Park, Eunmi Hong, Weontae Lee, Young Hwan Kim, Kun Kim, Young-Ki Paik |
Journal | Nature
(Nature)
Vol. 433
Issue 7025
Pg. 541-5
(Feb 03 2005)
ISSN: 1476-4687 [Electronic] England |
PMID | 15690045
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fatty Acids
- Pheromones
- daumone, C elegans
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Topics |
- Animals
- Caenorhabditis elegans
(drug effects, genetics, growth & development)
- Fatty Acids
(chemical synthesis, chemistry, isolation & purification, pharmacology)
- Larva
(drug effects, genetics, growth & development)
- Magnetic Resonance Spectroscopy
- Molecular Structure
- Pheromones
(chemical synthesis, chemistry, isolation & purification, pharmacology)
- Reproducibility of Results
- Signal Transduction
(drug effects)
- Stereoisomerism
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