Expression of apoptosis-related proteins in advanced uterine cervical cancer after balloon-occluded arterial infusion chemotherapy as an indicator of the efficiency of this therapy.

Abstract

We previously reported satisfactory therapeutic results of cisplatin-based cyclic balloon-occluded arterial infusion chemotherapy (BOAI) as neoadjuvant chemotherapy, which enabled treatment by hysterectomy in patients with advanced cervical cancer. We also reported expression of apoptosis among these patients and determined that the bax gene is related to this apoptosis. In the present study, we investigated the relationship between the effectiveness of BOAI therapy and expression of apoptosis regulatory genes and proteins in these cases. The subjects were 27 women with advanced cervical cancer classified as FIGO (International Federation of Gynecology and Obstetrics) stage III or higher who were admitted to the Department of Gynecology, Osaka City University Medical School Hospital between 2000 and 2003. All patients were treated by BOAI, and expression of cancer cell apoptosis was examined by the TUNEL method, expression of bax, bcl-2 and bcl-xL proteins were examined by immunohistochemistry, and expression of bax, bcl-2 and bcl-xL mRNA was examined by quantitative RT-PCR before and 3 days after BOAI. The effectiveness of BOAI therapy was thus determined. The 20 patients in whom BOAI was effective showed significantly higher expression of the bax protein and gene after BOAI, and cancer cell apoptosis was accelerated. On the other hand, the 7 patients in whom BOAI was ineffective showed significantly higher expression of the bcl-xL protein and gene after BOAI. These results suggest that bax/bcl-xL expression can be used as an indicator of the effectiveness of BOAI therapy.

• Authors: Eri Okamoto, Toshiyuki Sumi, Fumiko Misugi, Hiroyuki Nobeyama, Kanae Hattori, Hiroyuki Yoshida, Yoshinari Matsumoto, Tomoyo Yasui, Ken-Ichi Honda, Osamu Ishiko
• Journal: International journal of molecular medicine (Int J Mol Med) Vol. 15 Issue 1 Pg. 41-7 (Jan 2005) ISSN: 1107-3756 [Print] Greece
• PMID: 15583826 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BAX protein, human
• BCL2L1 protein, human
• Proto-Oncogene Proteins c-bcl-2
• RNA, Messenger
• bcl-2-Associated X Protein
• bcl-X Protein

Topics

• Adult
• Aged
• Apoptosis (drug effects, genetics)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Infusions, Intra-Arterial
• Middle Aged
• Neoplasm Staging
• Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Uterine Cervical Neoplasms (blood supply, drug therapy, metabolism, pathology)
• bcl-2-Associated X Protein
• bcl-X Protein