Wine
proteins play an important role in a wine's quality as they affect taste, clarity, and stability. To enhance our understanding of the
proteins in wine, nano-high-performance liquid chromatography (HPLC)/tandem mass spectrometry was used to profile soluble
proteins in wine. Twenty
proteins were identified from a Sauvignon Blanc wine including five
proteins derived from the grape, 12 from yeast, two from bacteria, and one from fungi. The findings are somewhat peculiar at first glance, but reasonable explanations can account for the results. The grape
proteins identified are less in number, which may be due to the availability of an incomplete database and possibly
bentonite fining. The relatively large number of identified
yeast proteins may be due to their complete protein database. The identified bacterial and
fungal proteins could possibly be attributed to sources in the vineyard including natural
infections and improper handling during harvest. The use of nano-HPLC/tandem mass spectrometry is an important tool for identifying wine
proteins and understanding how they affect its characteristics.