Humoral and delayed-type hypersensitive responses against listeria monocytogenes phosphatidylinositol-specific phospholipase C in experimentally infected buffaloes.
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| Abstract |
The kinetics of antibody production against phosphatidylinositol-specific phospholipase C (PI-PLC) and the isolation pattern of Listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3 x 10(9) cells each of pathogenic L. monocytogenes. Antibodies to PI-PLC appeared by 4-8 days post infection (PI), with a peak between days 7 and 16 PI, when tested by indirect plate-ELISA. Subsequently, antibody titres in all the animals declined and became undetectable on days 26-35 PI onwards until the study concluded on day 211 PI. Dot-ELISA could detect the antibodies to PI-PLC 1-2 days earlier and at higher titres as compared to plate-ELISA. L. monocytogenes could be recovered from faeces, nasal swabs and haemocultures from days 2 to 33, days 2 to 21 and days 11 to 17 PI, respectively. Antibodies to PI-PLC were detected during the course of active infection but their titres declined sharply once animals became culturally negative. Sonicated antigen elicited the highest delayed-type hypersensitivity response, followed by PI-PLC and listeriolysin O.
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| Authors | S P Chaudhari, S V S Malik, S B Barbuddhe |
| Journal | Veterinary research communications (Vet Res Commun) Vol. 28 Issue 7 Pg. 569-79 (Oct 2004) ISSN: 0165-7380 [Print] Netherlands |
| PMID | 15563104 (Publication Type: Journal Article) |
| Chemical References |
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