Effects of interleukin-5 on acute myeloid leukemias.

Abstract

Because of the known activity of interleukin-5 (IL-5) as a growth factor for human eosinophils, we performed studies to ascertain whether M4Eo acute leukemia cells might be capable of responding to IL-5. Surprisingly, short term incubation of freshly isolated M4Eo blasts with rhIL-5 induced differentiation of the cells to macrophages. To determine whether other variants of acute myelogenous leukemia (AML) might also be capable of responding to IL-5, we studied cells from four additional unselected cases and found that one responded to IL-5 by vigorous proliferation. Using biotinylated rhIL-5, second labelling with streptavidin-fluorescein isothiocyanate (FITC) and flow cytometric analysis, specific binding of IL-5 to both responsive leukemias was demonstrated, suggesting the presence of specific IL-5 receptors. Additional study will be needed to assess the frequency of IL-5 responsiveness in AML and to determine the relationship of these phenomena to the physiologic role of IL-5 in the regulation of hematopoiesis.

• Authors: M A Baumann, C C Paul, M J Grace
• Journal: American journal of hematology (Am J Hematol) Vol. 39 Issue 4 Pg. 269-74 (Apr 1992) ISSN: 0361-8609 [Print] United States
• PMID: 1553955 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Interleukin-5
• Fluorescein-5-isothiocyanate

Topics

• Cell Differentiation (drug effects)
• Cell Division (drug effects)
• Erythroblasts (drug effects, pathology)
• Flow Cytometry
• Fluorescein-5-isothiocyanate
• Humans
• Interleukin-5 (pharmacology, therapeutic use)
• Leukemia, Myeloid, Acute (blood)
• Leukemia, Myelomonocytic, Acute (blood)
• Tumor Cells, Cultured (drug effects, pathology)