[Expression of pRb and E2F-1 and telomerase activity in ameloblastoma].

Abstract

OBJECTIVE:

To investigate the expression of pRb and E2F-1, and the association between their expression and the activity of telomerase (hTERT) or cyclin E in human ameloblastoma (AB), and to explore the clinical biological characteristics of AB.

METHODS:

The expressions of pRb, E2F-1, cyclin E and hTERT mRNA in human AB were detected by in situ hybridization or immunohistochemistry (SP method).

RESULTS:

The positive expression ratio of pRb in the cell nucleus of AB was 20.4% (11/54). The positive ratio of E2F-1, cyclin E and hTERT mRNA was 92.6% (50/54), 66.7% (36/54) and 94.4% (51/54), respectively. With AB recurrence and malignant transformation, the expression of hTERT, E2F-1, cyclin E was up-regulated. hTERT and cyclin E or E2F-1 mRNA had high positive relation (Spearsman'r(s) = 1.000, P = 0.0001).

CONCLUSIONS:

The regulatory pathway of Rb/E2F-1 is associated with the cell proliferation and in differentiation of AB. The activity or release of telomerase may be related to the lower expression of Rb and higher expression of E2F-1, and is up-regulated in G(1) late phase by cyclin E.

• Authors: Ming Zhong, Jie Wang, Bo Zhang, Lin Hou, Yang-li Yue, Zi-juan Li
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 39 Issue 5 Pg. 406-9 (Sep 2004) ISSN: 1002-0098 [Print] China
• PMID: 15498350 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclin E
• E2F1 Transcription Factor
• RNA, Messenger
• Retinoblastoma Protein
• Telomerase

Topics

• Adolescent
• Adult
• Aged
• Ameloblastoma (metabolism, pathology)
• Child
• Cyclin E (biosynthesis)
• E2F1 Transcription Factor (biosynthesis)
• Female
• Humans
• Jaw Neoplasms (metabolism, pathology)
• Male
• Middle Aged
• RNA, Messenger (biosynthesis)
• Retinoblastoma Protein (biosynthesis)
• Telomerase (genetics, metabolism)