Vitamin D-interacting protein 205 (DRIP205) coactivation of estrogen receptor alpha (ERalpha) involves multiple domains of both proteins.

Abstract

Vitamin D-interacting protein 205 (DRIP205) is a mediator complex protein that anchors the complex to the estrogen receptor (ER) and other nuclear receptors (NRs). In ZR-75 breast cancer cells treated with 17beta-estradiol (E2) and transfected with a construct containing three tandem estrogen responsive elements (pERE(3)), DRIP205 coactivates ERalpha-mediated transactivation. DRIP205Delta587-636 is a DRIP205 mutant in which both NR boxes within amino acids 587-636 have been deleted and, in parallel transfection studies, DRIP205Delta587-636 also coactivates ERalpha. Moreover, both wild-type and variant DRIP205 also colocalize with ERalpha in the nuclei of transfected cells. Extensive deletion analysis of DRIP205 shows that multiple domains of this protein play a role in coactivation of ERalpha and in interactions with ERalpha. Coactivation of ERalpha by DRIP205 does not require NR boxes, and variants with deletion of N-terminal (amino acids 1-639) and C-terminal (amino acids 576-1566) significantly coactivate ERalpha. DRIP205 resembles p160 coactivators that also interact with multiple regions of ERalpha; however, unlike p160 coactivators, DRIP205 coactivation of ERalpha does not require NR boxes.

• Authors: Qian Wu, Robert Burghardt, Stephen Safe
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 279 Issue 51 Pg. 53602-12 (Dec 17 2004) ISSN: 0021-9258 [Print] United States
• PMID: 15471764 (Publication Type: Journal Article)

Chemical References

• Estrogen Receptor alpha
• Estrogens
• MED1 protein, human
• Mediator Complex Subunit 1
• Oligonucleotides
• Receptors, Estrogen
• Transcription Factors
• DNA Restriction Enzymes

Topics

• Animals
• Blotting, Western
• COS Cells
• Cell Line, Tumor
• Cell Nucleus (metabolism)
• Cloning, Molecular
• DNA Restriction Enzymes (pharmacology)
• Estrogen Receptor alpha (metabolism)
• Estrogens (metabolism)
• Gene Deletion
• Humans
• Mediator Complex Subunit 1
• Mutation
• Oligonucleotides (chemistry)
• Plasmids (metabolism)
• Protein Binding
• Protein Structure, Tertiary
• Receptors, Estrogen (metabolism)
• Response Elements
• Transcription Factors (metabolism)
• Transcriptional Activation
• Transfection