Human Arf tumor suppressor specifically interacts with chromatin containing the promoter of rRNA genes.

Abstract

The tumor suppressor Arf (Alternative Reading Frame) protein (p14ARF in human and p19ARF in mouse) is mainly located in the nucleolus consistent with its subcellular localization, the protein has been shown to specifically interact with 5.8S rRNA and with B23/Nucleophosmin and to regulate ribosome biogenesis. Here, we show that the p14ARF protein interacts with chromatin and is recovered by chromatin immunoprecipitation (ChIP) in a fraction that contains a DNA sequence of the rRNA gene promoter. In addition, topoisomerase I (Topo I) that has been shown to interact with p14ARF coprecipitates with p14ARF containing chromatin. These data, in view of the function for Topo I in rRNA transcription, are consistent with a role for the p14ARF-Topo I complex in rRNA transcription and/or maturation.

• Authors: Olivier Ayrault, Laetitia Andrique, Christian-Jacques Larsen, Paule Seite
• Journal: Oncogene (Oncogene) Vol. 23 Issue 49 Pg. 8097-104 (Oct 21 2004) ISSN: 0950-9232 [Print] England
• PMID: 15361825 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chromatin
• Tumor Suppressor Protein p14ARF
• DNA Topoisomerases, Type I

Topics

• Chromatin (metabolism)
• DNA Topoisomerases, Type I (metabolism)
• Genes, rRNA
• Humans
• Promoter Regions, Genetic
• Tumor Suppressor Protein p14ARF (physiology)