Abstract | BACKGROUND: METHODS: The nuclear envelope was extracted from human cadaver kidneys. 14C oxalate was labeled, nuclear pore complex proteins were extracted and loaded onto Sephadex G-200, and further purified in DEAE-Sephadex A-50 column. The radioactive protein peak was pooled, concentrated and checked for purity in SDS-PAGE. The purified protein showed cross-reactivity with the monoclonal antibody (MAb 414) and was homogeneous. Urine samples of healthy individuals with no history of kidney disease served as control. Blood and urine samples were collected from kidney and autoimmune disorder patients and checked for the expression of p62 protein by ELISA. RESULTS: CONCLUSION: Increased expression of p62 may be due to membrane damage induced by oxalate stress, and may be used as a diagnostic marker. This study also confirms the presence of p62 autoantibodies in HO patients.
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Authors | P Sivakamasundari, P Kalaiselvi, R Sakthivel, R Selvam, P Varalakshmi |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 347
Issue 1-2
Pg. 111-9
(Sep 2004)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 15313148
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Membrane Glycoproteins
- Nuclear Pore Complex Proteins
- nuclear pore protein p62
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Topics |
- Autoantibodies
(analysis)
- Blotting, Western
- Chromatography, Ion Exchange
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Humans
- Hyperoxaluria
(metabolism)
- In Vitro Techniques
- Kidney
(metabolism)
- Kidney Calculi
(metabolism)
- Kidney Diseases
(metabolism)
- Membrane Glycoproteins
(biosynthesis, isolation & purification, urine)
- Molecular Weight
- Nuclear Pore
(chemistry)
- Nuclear Pore Complex Proteins
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