The effects of the delta-opioid agonist SNC80 on hind-limb motor function and neuronal injury after spinal cord ischemia in rats.

Abstract

Recent investigation suggested neuroprotective efficacy of a delta-opioid agonist in the brain. We investigated the effects of intrathecal treatment with a delta-opioid agonist (SNC80) on spinal cord ischemia (SCI) in rats. SCI was induced with an intraaortic balloon catheter. The animals were randomly allocated to one of the following five groups: 1) SNC80 before 9 min of SCI (SNC-9; n = 12), 2) vehicle before 9 min of SCI (V-9; n = 12), 3) SNC80 before 11 min of SCI (SNC-11; n = 10), 4) vehicle before 11 min of SCI (V-11; n = 12), or 5) sham (n = 12). SNC80 (400 nmol) or vehicle was administered 15 min before SCI. Forty-eight hours after reperfusion, hind-limb motor function was assessed by using the Basso, Beattie, Bresnahan (BBB) scale (0 = paraplegia; 21 = normal) and histological assessment of the L4 and L5 spinal segments was performed. BBB scores in the SNC-9 group were higher compared with those in the V-9 group (P < 0.05), whereas there were no differences in BBB scores between the SNC-11 and V-11 groups. There were significantly more normal neurons in the SNC-9 and SNC-11 groups than in the V-9 and V-11 groups (P < 0.05). The results indicate that intrathecal treatment with the delta-opioid agonist SNC80 can attenuate hind-limb motor dysfunction and neuronal injury after SCI in rats.

• Authors: Toshinori Horiuchi, Masahiko Kawaguchi, Takanori Sakamoto, Naoko Kurita, Satoki Inoue, Mitsutoshi Nakamura, Noboru Konishi, Hitoshi Furuya
• Journal: Anesthesia and analgesia (Anesth Analg) Vol. 99 Issue 1 Pg. 235-240 (Jul 2004) ISSN: 0003-2999 [Print] United States
• PMID: 15281536 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzamides
• Neuroprotective Agents
• Piperazines
• Receptors, Opioid, delta
• 4-(alpha-(4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl)-N,N-diethylbenzamide

Topics

• Animals
• Benzamides (pharmacology)
• Blood Gas Analysis
• Body Temperature (physiology)
• Hindlimb (physiology)
• Injections, Spinal
• Male
• Movement (physiology)
• Neurons (drug effects, pathology)
• Neuroprotective Agents (pharmacology)
• Piperazines (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Receptors, Opioid, delta (agonists)
• Reperfusion Injury (pathology, prevention & control)
• Spinal Cord Ischemia (pathology)