A novel role for the immunophilin FKBP52 in copper transport.

Abstract

FK506-binding protein 52 (FKBP52) is an immunophilin that possesses peptidylprolyl cis/trans-isomerase (PPIase) activity and is a component of a subclass of steroid hormone receptor complexes. Several recent studies indicate that immunophilins can regulate neuronal survival and nerve regeneration although the molecular mechanisms are poorly understood. To investigate the function of FKBP52 in the nervous system, we employed a yeast two-hybrid strategy using the PPIase domain (domain I) as bait to screen a neonatal rat dorsal root ganglia cDNA expression library. We identified an interaction between FKBP52 domain I and Atox1, a copper-binding metallochaperone. Atox1 interacts with Menkes disease protein and Wilson disease protein (WD) and functions in copper efflux. The interaction between FKBP52 and Atox1 was observed in both glutathione S-transferase pull-down experiments and when proteins were ectopically expressed in human embryonic kidney (HEK) 293T cells and was sensitive to FK506. Interestingly, the FKBP52/Atox1 interaction was enhanced when HEK 293T cells were cultured in copper-supplemented medium and decreased in the presence of the copper chelator, bathocuproine disulfate, suggesting that the interaction is regulated in part by intracellular copper. Overexpression of FKBP52 increased rapid copper efflux in (64)Cu-loaded cells, as did the overexpression of WD transporter. Taken together, our present findings suggest that FKBP52 is a component of the copper efflux machinery, and in so, may also promote neuroprotection from copper toxicity.

• Authors: Reiko Sanokawa-Akakura, Huachang Dai, Shin Akakura, David Weinstein, J Eduardo Fajardo, Steven E Lang, Scott Wadsworth, John Siekierka, Raymond B Birge
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 279 Issue 27 Pg. 27845-8 (Jul 02 2004) ISSN: 0021-9258 [Print] United States
• PMID: 15133031 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Chelating Agents
• DNA, Complementary
• Phenanthrolines
• bathocuproine sulfonate
• Copper
• Glutathione Transferase
• Tacrolimus Binding Proteins
• tacrolimus binding protein 4
• Immunophilins
• Calcium

Topics

• Animals
• Biological Transport
• Blotting, Western
• Calcium (metabolism)
• Cell Line
• Cell Survival
• Chelating Agents (pharmacology)
• Copper (metabolism)
• DNA, Complementary (metabolism)
• Dose-Response Relationship, Drug
• Gene Library
• Genetic Vectors
• Glutathione Transferase (metabolism)
• Humans
• Immunophilins (metabolism)
• Kinetics
• Models, Genetic
• Neurons (metabolism)
• Phenanthrolines (pharmacology)
• Precipitin Tests
• Protein Structure, Tertiary
• Rats
• Tacrolimus Binding Proteins (physiology)
• Time Factors
• Two-Hybrid System Techniques