Abstract |
In order to investigate the relationship between lens opacities and the various modifications of lens proteins, we analyzed and compared the properties of lens proteins of 85-day old normal Wistar rats and the hereditary cataract model, ICR/f rats. The present study identified many differences between normal and mutant lens proteins. In the ICR/f mutant rats, the relative amounts of gamma-crystallin decreased and high molecular weight (HMW) protein increased. Racemization and isomerization of Asp-151 of alpha A-crystallin was observed in the mutant ICR/f rats, and Met-1 of alpha A-crystallin was oxidized to methionine sulfoxide. These modifications were not found in the age-matched normal rats. These tendencies are consistent with aged and cataractous human lenses.
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Authors | N Fujii, N Takeuchi, N Fujii, T Tezuka, K Kuge, T Takata, A Kamei, T Saito |
Journal | Amino acids
(Amino Acids)
Vol. 26
Issue 2
Pg. 147-52
(Mar 2004)
ISSN: 0939-4451 [Print] Austria |
PMID | 15042443
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Peptides
- alpha-Crystallin A Chain
- Aspartic Acid
- Methionine
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Topics |
- Aging
(physiology)
- Animals
- Aspartic Acid
(analogs & derivatives, chemistry, metabolism)
- Cataract
(genetics, metabolism)
- Disease Models, Animal
- Humans
- Isomerism
- Lens, Crystalline
(chemistry, metabolism)
- Methionine
(metabolism)
- Molecular Structure
- Oxidation-Reduction
- Peptides
(chemistry)
- Protein Processing, Post-Translational
- Rats
- Rats, Mutant Strains
- Rats, Wistar
- Time Factors
- alpha-Crystallin A Chain
(chemistry, metabolism, physiology)
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