Heterogeneous nuclear ribonucleoproteins F and H/H' show differential expression in normal and selected cancer tissues.

Abstract

The heterogeneous nuclear ribonucleoproteins (hnRNPs) F and H/H', containing the quasi-RNA recognition motif (qRRM) domains, are implicated in several steps of pre-mRNA processing and in cellular differentiation. We have compared a set of tissues and found striking differences in their levels of expression as well as in the nuclear versus the cytoplasmic distribution. Generally, hnRNP F is broadly expressed in many tissues with extremely strong expression in the prostate gland while hnRNP H/H' shows a more restricted degree of expression with low expression in some tissues, for example, liver, exocrine acini of the pancreas, thyroid gland and heart. At the cellular level, hnRNP F is, with few exceptions, predominantly expressed in the cytoplasm while hnRNP H/H' is more abundant in the nuclei. A quite pronounced heterogeneous expression pattern is seen in the proximal tubules of the kidney where hnRNP F is present at moderate cytoplasmic levels while hnRNP H/H' is undetectable, whereas both proteins are more evenly expressed in distal tubules and collecting ducts. Generally, tumor tissues reveal a broad expression of hnRNP F in the nuclei as well as in the cytoplasm while hnRNP H/H' is expressed at higher levels in the nuclei than in the cytoplasm. Up-regulation of hnRNP H/H' is found in a few tissues that normally express low cytoplasmic levels of hnRNP H/H', for example, adenocarcinoma of the pancreas, hepatocellular carcinoma and gastric carcinoma. hnRNP F is down-regulated in hepatocellular carcinoma and up-regulated in gastric carcinoma. The present study indicates the important potential role of this subset of hnRNPs on the gene expression in many tissues.

• Authors: Bent Honoré, Ulrik Baandrup, Henrik Vorum
• Journal: Experimental cell research (Exp Cell Res) Vol. 294 Issue 1 Pg. 199-209 (Mar 10 2004) ISSN: 0014-4827 [Print] United States
• PMID: 14980514 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Heterogeneous-Nuclear Ribonucleoprotein Group F-H

Topics

• Adenocarcinoma (metabolism, pathology)
• Carcinoma, Hepatocellular (metabolism, pathology)
• Heterogeneous-Nuclear Ribonucleoprotein Group F-H (immunology, metabolism)
• Humans
• Immunohistochemistry
• Liver Neoplasms (metabolism, pathology)
• Neoplasms (metabolism, pathology)
• Pancreatic Neoplasms (metabolism, pathology)
• Tissue Distribution
• Tumor Cells, Cultured