Evaluation of enzyme immunoassay using a recombinant envelope protein expressed in insect cells for serological confirmation of HTLV-I infection.
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| Abstract |
A recombinant human T-lymphotropic virus type I (HTLV-I) envelope protein expressed in insect cells using a recombinant baculovirus was employed as the antigen in an enzyme immunoassay (renvEIA). Peripheral blood samples were obtained from asymptomatic carriers or healthy individuals. Plasma was tested for HTLV-I antibody by renvEIA, particle agglutination, and Western immunoblot (WB), and lymphocyte DNA was tested for HTLV-I proviral DNA amplification by polymerase chain reaction (PCR). Of 61 people aged 9 months or older, 23 were positive (gag+, env+) and 19 others were in the "indeterminate" category (gag+, env-) when their WB results were interpreted according to the WHO-proposed criteria. Thirty-seven cases, including all of the WB+ cases and 14 of 19 WB indeterminate cases, were positive by renvEIA. In 34 of 37 renvEIA-positive cases, the presence of long terminal repeat (LTR) and tax/rex region of HTLV-I proviral DNA was detected by polymerase chain reaction (PCR) and following Southern blot hybridization. Thus, renvEIA would be a useful supplemental assay to confirm the presence of HTLV-I antibody in HTLV-I asymptomatic carriers.
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| Authors | K Yamashita, M Maekawa, K Mitani, M Wakamiya, T Ogino, K Miyamura, K Baba, Y Yamamoto, H Nyunoya, K Shimotohno |
| Journal | AIDS research and human retroviruses (AIDS Res Hum Retroviruses) Vol. 8 Issue 11 Pg. 1857-61 (Nov 1992) ISSN: 0889-2229 [Print] United States |
| PMID | 1489575 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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