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C1-esterase inhibitor and a novel peptide inhibitor improve contractile function in reperfused skeletal muscle.

Abstract
To determine the role of inhibition of complement activation in the contractile function of skeletal muscle ischemia-reperfusion (I/R) injury, the rat extensor digitorum longus (EDL) muscles underwent 3 h ischemia and received human C1-esterase inhibitor (C1-INH, 100 IU/kg), a synthetic C1q A chain peptide with a similar inhibitory effect on activated C1 (peptide, 5 mg/kg), or human serum albumin control. Results showed a significant overall increase in tetanic contractile forces of the reperfused EDL in both C1-INH and peptide groups compared to controls. Maximum improvement occurred with peptide treatment at 120-Hz stimulation, with an increase in force from 38 +/- 4% of normal in controls to 52 +/- 4% in peptide-treated rats. There were no significant differences between C1-INH and peptide groups. Plasma C3 and C4 activities were significantly increased in both treated groups, suggesting inhibition of complement activation. Our results suggest that complement activation is involved in I/R injury, and inhibition of complement activation may therefore represent a potential therapeutic approach to reducing or preventing I/R injury.
AuthorsGlen A Toomayan, Long-En Chen, Hai-Xiang Jiang, Wen-Ning Qi, Anthony V Seaber, Michael M Frank, James R Urbaniak
JournalMicrosurgery (Microsurgery) Vol. 23 Issue 6 Pg. 561-7 ( 2003) ISSN: 0738-1085 [Print] United States
PMID14705072 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
CopyrightCopyright 2003 Wiley-Liss, Inc.
Chemical References
  • Complement C1 Inactivator Proteins
  • Complement C1q
Topics
  • Animals
  • Complement Activation
  • Complement C1 Inactivator Proteins (pharmacology)
  • Complement C1q (pharmacology)
  • Disease Models, Animal
  • Female
  • Humans
  • Muscle Contraction (drug effects, physiology)
  • Muscle, Skeletal (drug effects, physiology)
  • Probability
  • Rats
  • Rats, Sprague-Dawley
  • Reference Values
  • Reperfusion Injury (drug therapy, pathology)
  • Sensitivity and Specificity

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