[Expression of telomerase and p53 in ameloblastoma].

Abstract

OBJECTIVE:

To detect the expression of p53 protein, hTR, and hTERT mRNA in ameloblastoma (AB) and to investigate the clinical biological characteristics of AB.

METHODS:

We detected hTR and hTERT Mrna in 54 cases of AB by in situ hybridization, and p53 protein by S-P method.

RESULTS:

The positive ratio of hTR, hTERT mRNA was 2/7 and 1/7 in normal oral mucosa, 81.2%(13/16) and 87.4% (14/16) in odontogenic keratocyst (OKC), 94.4%(51/54) in AB, respectively. There was a significant difference in these three groups (P < 0.001). A strong correlation between hTR and hTERT mRNA was found (rs = 1.000, P < 0.001). p53 protein was expressed in 87.5% (42/49) AB, 44%(11/25) OKC, 33.3%(1/3) normal oral mucosa, respectively. A strong correlation between hTERT mRNA and p53 protein was found (rs = 0.848, P < 0.001). A moderate correlation between hTR mRNA and p53 protein was found (rs = 0.536, P < 0.001).

CONCLUSION:

The positive ratios of hTR and hTERT were higher than p53 protein in AB. Telomerase activity and p53 expression play an important role in genesis and development of AB.

• Authors: Ming Zhong, Lu-huang Zhang, Jie Wang, Bo Zhang, Lin Hou
• Journal: Shanghai kou qiang yi xue = Shanghai journal of stomatology (Shanghai Kou Qiang Yi Xue) Vol. 12 Issue 2 Pg. 127-31 (Apr 2003) ISSN: 1006-7248 [Print] China
• PMID: 14661519 (Publication Type: English Abstract, Journal Article)

Chemical References

• DNA-Binding Proteins
• RNA, Messenger
• Tumor Suppressor Protein p53
• Telomerase

Topics

• Adolescent
• Adult
• Aged
• Ameloblastoma (chemistry, etiology)
• Child
• DNA-Binding Proteins
• Female
• Humans
• Immunohistochemistry
• In Situ Hybridization
• Jaw Neoplasms (chemistry, etiology)
• Male
• Middle Aged
• Odontogenic Cysts (metabolism)
• RNA, Messenger (analysis)
• Telomerase (analysis, genetics)
• Tumor Suppressor Protein p53 (analysis, genetics)