Protection of mice against experimental Japanese encephalitis virus infections by neutralizing anti-glycoprotein E monoclonal antibodies.

Abstract

Neutralizing monoclonal antibodies (MAbs) to glycoprotein E (gpE) of Japanese encephalitis (JE) virus given intraperitoneally (i.p.) (0.1 ml of immune ascitic fluid (AF) diluted 1:10 per mouse) to about 4-week-old Swiss mice 1 day prior or 2 days after the virus challenge (100 LD50 of JE virus administered intracerebrally (i.c.)) resulted in a decreased mortality along with an increased survival of the animals as demonstrated by the HAI-positive virus-specific (Hs) MAbs. The protective effect produced by four Hs MAbs was maximum when given 1 day prior the virus challenge, while other, namely HAI-positive flavivirus cross-reactive (Hx) and HAI-negative virus-specific (NHs) MAbs did not produce any effect. Interestingly, one of the two NHs MAbs, namely NHs-1 showed a reduced survival of mice given the MAb 2 days after the virus challenge. Administration of combinations of two or more Hs MAbs may be recommended due to their possible enhanced protection against JE virus infections in mice.

• Authors: A K Gupta, V J Lad, A A Koshy
• Journal: Acta virologica (Acta Virol) Vol. 47 Issue 3 Pg. 141-5 ( 2003) ISSN: 0001-723X [Print] Slovakia
• PMID: 14658841 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Immune Sera
• Membrane Glycoproteins
• Viral Envelope Proteins
• glycoprotein E, Japanese encephalitis virus

Topics

• Animals
• Antibodies, Monoclonal (administration & dosage, immunology)
• Antibodies, Viral (administration & dosage, immunology)
• Encephalitis Virus, Japanese (immunology, pathogenicity)
• Encephalitis, Japanese (mortality, prevention & control)
• Humans
• Immune Sera (administration & dosage, immunology)
• Immunization, Passive
• Membrane Glycoproteins (immunology)
• Mice
• Mice, Inbred BALB C
• Neutralization Tests
• Viral Envelope Proteins (immunology)