1,25-dihydroxyvitamin D3 and development of tuberculosis in cattle.

Abstract

This report describes the presence and activity of 1,25-dihydroxyvitamin D3 (1,25-D3) in experimental bovine tuberculosis. Animals that went on to develop tuberculous lesions exhibited a rapid transient increase in serum 1,25-D3 within the first 2 weeks following infection with Mycobacterium bovis. 1,25-D3-positive mononuclear cells were later identified in all tuberculous granulomas by immunohistochemical staining of postmortem lymph node tissue. These results suggest a role for 1,25-D3 both at the onset of infection and in the development of the granuloma in these infected animals. Using a monoclonal antibody to the vitamin D receptor (VDR) as a VDR agonist, we confirmed that activation of the vitamin D pathway profoundly depresses antigen-specific, but not mitogenic, bovine peripheral blood T-cell responses (proliferation and gamma interferon production). Investigation of the mechanism of this suppression showed that the VDR antibody modified the expression of CD80 by accessory cells, such that a significant positive correlation between T-cell proliferation and accessory cell CD80 emerged.

• Authors: S G Rhodes, L A Terry, J Hope, R G Hewinson, H M Vordermeier
• Journal: Clinical and diagnostic laboratory immunology (Clin Diagn Lab Immunol) Vol. 10 Issue 6 Pg. 1129-35 (Nov 2003) ISSN: 1071-412X [Print] United States
• PMID: 14607878 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• B7-1 Antigen
• Calcitriol

Topics

• Animals
• B7-1 Antigen (analysis)
• Calcitriol (analysis, physiology)
• Cattle
• Granuloma (etiology)
• Leukocytes, Mononuclear (chemistry)
• Lymph Nodes (pathology)
• Lymphocyte Activation (immunology)
• Mycobacterium bovis
• T-Lymphocytes (immunology)
• Tuberculosis, Bovine (metabolism)