Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro.

Abstract

AIM:

To study the inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma(HCC) cells in vitro.

METHODS:

Antisense RNA of HAb18G/CD147 vector PCI-asHAb18G was constructed by reversely inserting HAb18G/CD147 cDNA to eukaryotic expression vector PCI-neo. The HCC cell line HHCC was transfected by PCI-asHAb18G via cation liposome. Expression of HAb18G/CD147 of transfected cells selected by G418 (geneticin) was observed by immuno-histochemical SP staining and FACS (fluorescence activated cell sorting). Gelatin zymography was used to determine the effect of PCI-asHAb18G on reducing secretions of MMP-2 and MMP-9 of the transfected cells. Boyden chamber was employed to test the invasion of HCC cells in vitro.

RESULTS:

The construction of antisense RNA vector PCI-asHAb18G was verified correct by partial nucleotide sequencing and restricted endonuclease digestion. The expression of HAb18G/CD147 in transfected HHCC was inhibited by PCI-asHAb18G. Secretions of MMP-2 and MMP-9 of transfected HHCC were reduced and the invasion of transfected HHCC was inhibited compared to HHCC, respectively.

CONCLUSION:

Invasion of HCC cells can be inhibited by antisense RNA of HAb18G/CD147. HAb18G/CD147 may be used as a potential target of drugs for anti-invasion and metastasis of HCC.

• Authors: Yu Li, Peng Shang, Ai-Rong Qian, Li Wang, Yong Yang, Zhi-Nan Chen
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 9 Issue 10 Pg. 2174-7 (Oct 2003) ISSN: 1007-9327 [Print] United States
• PMID: 14562372 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Antigens, Neoplasm
• Antigens, Surface
• Avian Proteins
• BSG protein, human
• Blood Proteins
• Bsg protein, Gallus gallus
• Bsg protein, rat
• Membrane Glycoproteins
• RNA, Antisense
• Basigin
• Gelatin
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Antigens, CD
• Antigens, Neoplasm
• Antigens, Surface
• Avian Proteins
• Basigin
• Blood Proteins
• Carcinoma, Hepatocellular
• Cell Line, Tumor
• Cloning, Molecular
• Flow Cytometry
• Gelatin
• Humans
• Immunohistochemistry
• In Vitro Techniques
• Liver Neoplasms
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Membrane Glycoproteins (genetics)
• Neoplasm Invasiveness
• RNA, Antisense (pharmacology)
• Transfection