Expansion of the mammalian 3 beta-hydroxysteroid dehydrogenase/plant dihydroflavonol reductase superfamily to include a bacterial cholesterol dehydrogenase, a bacterial UDP-galactose-4-epimerase, and open reading frames in vaccinia virus and fish lymphocystis disease virus.

Abstract

Mammalian 3 beta-hydroxysteroid dehydrogenase and plant dihydroflavonol reductases are descended from a common ancestor. Here we present evidence that Nocardia cholesterol dehydrogenase, E. coli UDP-galactose-4 epimerase, and open reading frames in vaccinia virus and fish lymphocystis disease virus are homologous to 3 beta-hydroxysteroid dehydrogenase and dihydroflavonol reductase. Analysis of a multiple alignment of these sequences indicates that viral ORFs are most closely related to the mammalian 3 beta-hydroxysteroid dehydrogenases. The ancestral protein of this superfamily is likely to be one that metabolized sugar nucleotides. The sequence similarity between 3 beta-hydroxysteroid dehydrogenase and the viral ORFs is sufficient to suggest that these ORFs have an activity that is similar to 3 beta-hydroxysteroid dehydrogenase or cholesterol dehydrogenase, although the putative substrates are not yet known.

• Authors: M E Baker, R Blasco
• Journal: FEBS letters (FEBS Lett) Vol. 301 Issue 1 Pg. 89-93 (Apr 13 1992) ISSN: 0014-5793 [Print] England
• PMID: 1451793 (Publication Type: Journal Article)

Chemical References

• Viral Proteins
• Oxidoreductases
• 3-Hydroxysteroid Dehydrogenases
• Alcohol Oxidoreductases
• dihydroflavanol 4-reductase
• cholesterol dehydrogenase
• UDPglucose 4-Epimerase

Topics

• 3-Hydroxysteroid Dehydrogenases (genetics)
• Alcohol Oxidoreductases (genetics)
• Amino Acid Sequence
• Biological Evolution
• Iridoviridae (genetics)
• Molecular Sequence Data
• Multigene Family (genetics)
• Nocardia (enzymology, genetics)
• Oxidoreductases (genetics)
• Phylogeny
• Plants (enzymology, genetics)
• Sequence Homology, Amino Acid
• UDPglucose 4-Epimerase (genetics)
• Vaccinia virus (genetics)
• Viral Proteins (genetics)