Chondrocytic differentiation of peripheral neuroectodermal tumor cell line in nude mouse xenograft.

Abstract

We have established a cell line (KU-SN) from a peripheral neuroectodermal tumor originating in the left scapula of a 4-year-old girl. The original tumor was immunoreactive with antibodies for neurofilament proteins, neuron-specific enolase, vimentin, S100 protein, and beta 2-microglobulin. Dense core granules, 50-150 nm in diameter, were identified by electron microscopy. The cell line was established from tumor cells in metastatic lung fluid. KU-SN cells were immunoreactive with the antibodies for neurofilament proteins, vimentin, neuron-specific enolase, S100 protein, glial fibrillary acidic protein, cytokeratin, and carcinoembryonic antigen. Besides these neuronal features, KU-SN cells express type 2 collagen and insulin-like growth factor 1 receptor. The addition of insulin-like growth factor 1 (100 ng/ml) increased the growth rate of KU-SN cells 2.1-fold over control. Some cells were positive for Alcian blue and alkaline phosphatase staining. Cytogenetic analysis of KU-SN cells disclosed a reciprocal chromosomal translocation [t(11,22)]. Northern blot analysis of KU-SN cells demonstrated amplified expression of the c-myc gene but not the N-myc gene. When tumor cells were transplanted into nude mice, cartilage was formed. The cartilage was immunoreactive with the antibody for HLA-ABC, indicating that it was derived from the tumor cells, not from mouse tissue. Chondrocytic differentiation was not observed in xenografts of Ewing's sarcoma cell lines SK-ES or RD-ES or the peripheral neuroectodermal tumor cell line SK-N-MC. These results indicate that KU-SN cells represent primitive neural crest cells having the potential for chondrocytic differentiation.

• Authors: J Goji, K Sano, H Nakamura, H Ito
• Journal: Cancer research (Cancer Res) Vol. 52 Issue 15 Pg. 4214-20 (Aug 01 1992) ISSN: 0008-5472 [Print] United States
• PMID: 1379122 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neurofilament Proteins
• Oligodeoxyribonucleotides
• RNA, Messenger
• Transforming Growth Factor beta
• RNA
• Insulin-Like Growth Factor I
• Collagen

Topics

• Animals
• Base Sequence
• Bone Neoplasms (genetics, pathology, ultrastructure)
• Cartilage (cytology, pathology)
• Cell Differentiation
• Cell Division (drug effects)
• Cell Line
• Child, Preschool
• Chromosomes, Human, Pair 11
• Chromosomes, Human, Pair 22
• Collagen (genetics)
• Culture Techniques (methods)
• Cytoplasmic Granules (ultrastructure)
• Exons
• Female
• Genes, myc
• Humans
• Immunohistochemistry
• Insulin-Like Growth Factor I (pharmacology)
• Mice
• Mice, Nude
• Molecular Sequence Data
• Neoplasm Transplantation
• Neoplasms, Germ Cell and Embryonal (genetics, pathology, ultrastructure)
• Neurofilament Proteins (analysis)
• Oligodeoxyribonucleotides
• Polymerase Chain Reaction (methods)
• RNA (genetics, isolation & purification)
• RNA, Messenger (analysis, genetics)
• Transforming Growth Factor beta (pharmacology)
• Translocation, Genetic
• Transplantation, Heterologous