Reductase activity encoded by the HM1 disease resistance gene in maize.

Abstract

The HM1 gene in maize controls both race-specific resistance to the fungus Cochliobolus carbonum race 1 and expression of the NADPH (reduced form of nicotinamide adenine dinucleotide phosphate)-dependent HC toxin reductase (HCTR), which inactivates HC toxin, a cyclic tetrapeptide produced by the fungus to permit infection. Several HM1 alleles were generated and cloned by transposon-induced mutagenesis. The sequence of wild-type HM1 shares homology with dihydroflavonol-4-reductase genes from maize, petunia, and snap-dragon. Sequence homology is greatest in the beta alpha beta-dinucleotide binding fold that is conserved among NADPH- and NADH (reduced form of nicotinamide adenine dinucleotide)-dependent reductases and dehydrogenases. This indicates that HM1 encodes HCTR.

• Authors: G S Johal, S P Briggs
• Journal: Science (New York, N.Y.) (Science) Vol. 258 Issue 5084 Pg. 985-7 (Nov 06 1992) ISSN: 0036-8075 [Print] United States
• PMID: 1359642 (Publication Type: Journal Article)

Chemical References

• Peptides, Cyclic
• Plant Proteins
• RNA, Messenger
• NADP
• HC toxin
• DNA
• HC toxin reductase, Zea mays
• Oxidoreductases

Topics

• Amino Acid Sequence
• Base Sequence
• Blotting, Southern
• Cloning, Molecular
• DNA (chemistry, genetics)
• Genes, Plant
• Helminthosporium
• Introns
• Molecular Sequence Data
• NADP (pharmacology)
• Nucleic Acid Hybridization
• Oxidoreductases (chemistry, genetics)
• Peptides, Cyclic (antagonists & inhibitors)
• Plant Diseases
• Plant Proteins
• Polymorphism, Restriction Fragment Length
• RNA Splicing
• RNA, Messenger (genetics)
• Zea mays (enzymology, genetics)