Expression of LFA-1/ICAM-1 in CNS lymphomas: possible mechanism for lymphoma homing into the brain.

Abstract

We examined a possible role for the adhesion molecules LFA-1 and ICAM-1 in localizing central nervous system non-Hodgkin's lymphomas (CNS-NHLs) to the brain. Fresh frozen sections from 12 monoclonal CNS NHLs (11 primary, one secondary) were stained with monoclonal antibodies to LFA-1 alpha chain (CD11a), beta chain (CD18) and, ICAM-1 (CD54). Additional staining made use of rat monoclonal antibodies to the human and mouse high endothelial venule antigens HECA 452 and MECA 79 and mouse ICAM-1. The expression of these same molecules was also studied in mice with severe combined immunodeficiency (SCID) mice, bearing intracranial human lymphoblastoid cells. Eleven of the CNS-NHL tumors expressed LFA-1 alpha (one strongly, one intermediate, nine weakly). Nine of the tumors weakly expressed LFA-1 beta.. Nine of twelve tumors weakly expressed ICAM-1. In six of seven tumors definite blood vessels stained for ICAM-1. Non-tumor brain from two patients and non-tumor cerebral blood vessels showed no staining with CD11a, CD18 or CD54 antibodies. Strong expression of LFA-alpha and LFA-beta as well as ICAM-1 was noted in human lymphoblastoid cells (LCLs)/SCID mouse CNS lymphomas. Tumor blood vessels in these mice stained for mouse ICAM-1. Normal SCID mouse brains showed no staining with CD11a, CD18, CD54 or mouse ICAM-1 antibodies. Human, human/mouse CNS lymphomas, normal human, and mouse brains showed no staining with either HECA 452 or MECA 79.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: R Bashir, H Coakham, F Hochberg
• Journal: Journal of neuro-oncology (J Neurooncol) Vol. 12 Issue 2 Pg. 103-10 (Feb 1992) ISSN: 0167-594X [Print] United States
• PMID: 1348529 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Cell Adhesion Molecules
• Lymphocyte Function-Associated Antigen-1
• Intercellular Adhesion Molecule-1

Topics

• Animals
• Antigens, CD (metabolism, physiology)
• Brain Neoplasms (metabolism)
• Cell Adhesion Molecules (metabolism, physiology)
• Chimera (physiology)
• Female
• Humans
• Intercellular Adhesion Molecule-1
• Lymphocyte Function-Associated Antigen-1 (metabolism, physiology)
• Lymphoma, Large B-Cell, Diffuse (metabolism)
• Male
• Mice
• Mice, SCID
• Organ Specificity (physiology)
• Reference Values