Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections.
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| Abstract |
We have found that NS1 serotype-specific immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) can be used to differentiate primary and secondary dengue virus infections. This is due to the fact that the NS1-specific IgG antibody cannot be detected before day 9 of illness for primary infection, so the NS1-specific IgG antibodies measured in acute-phase sera must come from previous infection. Comparison of NS1 serotype-specific IgG ELISA with envelope- and membrane-specific capture IgM and IgG ELISA in the differentiation of primary and secondary dengue virus infections showed good correlation (95.90% agreement). Most important, we have found that the serotype of the dengue virus from the majority of patients with primary infection could be correctly identified when convalescent-phase or postinfection sera were analyzed by NS1 serotype-specific IgG ELISA. These findings suggested that NS1 serotype-specific IgG ELISA could be reliably applied for serodiagnosis and seroepidemiological study of dengue virus infection.
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| Authors | Pei-Yun Shu, Li-Kuang Chen, Shu-Fen Chang, Yi-Yun Yueh, Ling Chow, Li-Jung Chien, Chuan Chin, Ting-Hsiang Lin, Jyh-Hsiung Huang |
| Journal | Clinical and diagnostic laboratory immunology (Clin Diagn Lab Immunol) Vol. 10 Issue 4 Pg. 622-30 (Jul 2003) ISSN: 1071-412X [Print] United States |
| PMID | 12853395 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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