Abstract |
We examined the eIF-5A protein expression in 93 lung adenocarcinomas and 10 uninvolved lung samples using quantitative two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis with identification by mass spectrometry and 2-D Western blots. The same tissue samples were examined for the eIF-5A mRNA expression using oligonucleotide microarrays, and the cellular localization of the eIF-5A protein was examined using immunohistochemical analysis on tissue arrays. Higher eIF-5A protein expression was present in tumors showing poor differentiation, 12/13(th) codon K-ras mutations, p53 nuclear accumulation, and tumors with positive lymphocytic response. The eIF-5A mRNA was also significantly increased in lung adenocarcinomas compared to normal lung, but the eIF-5A protein expression was not correlated to its mRNA levels indicating that the increase in the eIF-5A protein expression in lung tumors is post-transcriptionally/translationally/post-translationally regulated. Patients having a higher eIF-5A protein expression showed a relatively poorer survival suggesting the use of eIF-5A as prognostic marker in lung adenocarcinoma. Moreover, the investigation on agents that inhibit eIF-5A function is encouraged.
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Authors | Guoan Chen, Tarek G Gharib, Dafydd G Thomas, Chiang-Ching Huang, David E Misek, Rork D Kuick, Thomas J Giordano, Mark D Iannettoni, Mark B Orringer, Samir M Hanash, David G Beer |
Journal | Proteomics
(Proteomics)
Vol. 3
Issue 4
Pg. 496-504
(Apr 2003)
ISSN: 1615-9853 [Print] Germany |
PMID | 12687616
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Interferon-alpha
- Interleukin-6
- Peptide Initiation Factors
- RNA, Messenger
- RNA-Binding Proteins
- Tumor Suppressor Protein p53
- eukaryotic translation initiation factor 5A
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Topics |
- Adenocarcinoma
(metabolism, pathology)
- Blotting, Western
- Cell Differentiation
- Cell Division
- Cell Line, Tumor
- Electrophoresis, Gel, Two-Dimensional
- Gene Expression Regulation, Neoplastic
- Genes, ras
(genetics)
- Humans
- Immunohistochemistry
- Interferon-alpha
(metabolism)
- Interleukin-6
(pharmacology)
- Lung Neoplasms
(metabolism, pathology)
- Mutation
- Oligonucleotide Array Sequence Analysis
- Peptide Initiation Factors
(analysis, chemistry, genetics, metabolism)
- Protein Biosynthesis
- Proteomics
- RNA, Messenger
(genetics, metabolism)
- RNA-Binding Proteins
- Survival Rate
- Tumor Suppressor Protein p53
(metabolism)
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