Column chromatographic analysis of relationship between hypertension-inducing potency and renin content of kidney extract.

Abstract

The relationship between hypertension-inducing potency and renin content of kidney extract was analyzed in rats using Sephadex G-100, CM-Sephadex C-50, DEAE-Cellulose or Concanavalin A-Sepharose column chromatography. Hypertension-inducing potency of each fraction obtained was evaluated in the basis of the final blood pressure level attained by repeated injections into the test animals for 10 days. Hypertension-inducing potency was found mainly in the renin-contaning fractions. And it seems reasonable to conclude that renin is implicated in the pathogenesis of hypertension produced by kidney extract. However, there were significant discrepancies between hypertension-inducing potency and renin content of subdivided fractions of these renin-containing eluates. Possible explantations for the discrepancies disclosed--including the possibility of the involvement of an unknown substance(s)--have been discussed. In addition, it was suggested from the results of Concanavalin A-Sepharose chromatography that rat renal renin has a glycoprotein nature.

• Authors: J Yamamoto, J Kira, M Matsunaga, K Ogino, C Kawai
• Journal: Japanese circulation journal (Jpn Circ J) Vol. 40 Issue 1 Pg. 21-8 (Jan 1976) ISSN: 0047-1828 [Print] Japan
• PMID: 1263330 (Publication Type: Journal Article)

Chemical References

• Renin

Topics

• Animals
• Blood Pressure
• Chromatography, Agarose
• Chromatography, DEAE-Cellulose
• Chromatography, Gel
• Female
• Hypertension (etiology)
• Kidney (analysis)
• Rats
• Rats, Inbred Strains
• Renin (isolation & purification)