A procedure for pulmonary lavage in mice.

Abstract

A method for the pulmonary lavage of mice is described. The procedure includes exsanguination of anesthetized mice by severting the renal artery, inserting a tracheal catheter in situ, and repeatedly injecting and aspirating 0.9% sodium chloride solution. Protein was recovered from the cell-free lavage fluid even after a given mouse was lavaged several times. The major part of the protein, however, was obtained with 1-ml washes repeated 3 times. Approximately 0.563 mg of protein was recovered by the procedure from a 29-g mouse. Four lavages per mouse yielded approximately 2.9 x 106 free cells.

• Authors: N I Medin, J W Osebold, Y C Zee
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 37 Issue 2 Pg. 237-8 (Feb 1976) ISSN: 0002-9645 [Print] United States
• PMID: 1259218 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Proteins

Topics

• Animals
• Catheterization (veterinary)
• Lung
• Proteins (isolation & purification)
• Therapeutic Irrigation (methods, veterinary)