Heat-induced superaggregation of amphotericin B attenuates its ability to induce cytokine and chemokine production in the human monocytic cell line THP-1.

Abstract	The cytokine and chemokine response elicited by heat-treated amphotericin B (HT-AmB) was compared with that of untreated amphotericin B (AmB-DOC) in the human monocyte cell line THP-1. AmB-DOC produced dose-dependent increases in interleukin (IL)-1beta, IL-1alpha, tumour necrosis factor-alpha, macrophage inflammatory protein (MIP)-1alpha and MIP-1beta at 2 h. HT-AmB induced cytokine and chemokine production at a lower level than those observed with corresponding concentrations of AmB-DOC, while retaining antifungal activity. These results indicate that heat treatment of amphotericin B may prove to be a cost-effective approach to improving the therapeutic index of this antifungal agent.
Authors	P David Rogers, Katherine S Barker, Vanessa Herring, Melissa Jacob
Journal	The Journal of antimicrobial chemotherapy (J Antimicrob Chemother) Vol. 51 Issue 2 Pg. 405-8 (Feb 2003) ISSN: 0305-7453 [Print] England
PMID	12562711 (Publication Type: Journal Article)
Chemical References	Anti-Bacterial Agents Chemokines Cytokines Indicators and Reagents Amphotericin B
Topics	Amphotericin B (pharmacology, toxicity) Anti-Bacterial Agents (pharmacology, toxicity) Cell Aggregation (drug effects) Cell Line Chemokines (biosynthesis) Cytokines (biosynthesis) Fungi (drug effects) Hot Temperature Humans Indicators and Reagents Microbial Sensitivity Tests Monocytes (metabolism)

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