Fat compartments and apolipoprotein B-100 kinetics in overweight-obese men.

Abstract

OBJECTIVE:

To examine the association between the kinetics of very-low-density-lipoprotein (VLDL)-apolipoprotein B-100 (apoB) and intraperitoneal, retroperitoneal, subcutaneous abdominal, and total adipose tissue masses (IPATM, RPATM, SAATM, and TATM, respectively) in overweight/obese men.

RESEARCH METHODS AND PROCEDURES:

Hepatic secretion of VLDL was measured using an intravenous infusion of 1-[(13)C]-leucine in 51 men with a wide range of body mass index (25.1 to 42.2 kg/m(2)). Isotopic enrichment of VLDL-apoB was measured using gas chromatography-mass spectrometry and a multicompartmental model used to estimate VLDL-apoB metabolic parameters. IPATM, RPATM, and SAATM (kilograms) were quantified between T11 and S1 using magnetic resonance imaging; TATM (kilograms) was determined using bioelectrical impedance. Insulin resistance was estimated by homeostasis model assessment (HOMA) score.

RESULTS:

In stepwise regression, IPATM was the best predictor of hepatic secretion of VLDL-apoB (r = 0.390, p < 0.005) and TATM was the best predictor of VLDL-apoB fractional catabolic rate (r = 0.282, p < 0.05). IPATM remained significantly associated with VLDL-apoB secretion after adjusting for TATM or HOMA score (r = 0.360, p < 0.01 and r = 0.310, p < 0.05, respectively). This association was also independent of age, dietary intake, and body mass index. None of the fat compartments were significantly associated with the fractional catabolic rate of VLDL-apoB after adjusting for HOMA score.

DISCUSSION:

In overweight/obese men, the quantity of both IPATM and TATM determine the kinetics of VLDL-apoB. The effect of IPATM on VLDL-apoB secretion is independent of both total fat mass and the degree of insulin resistance.

• Authors: Gerald F Watts, Dick C Chan, P Hugh R Barrett, Andrei V Susekov, Jianmin Hua, Swithin Song
• Journal: Obesity research (Obes Res) Vol. 11 Issue 1 Pg. 152-9 (Jan 2003) ISSN: 1071-7323 [Print] United States
• PMID: 12529498 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Apolipoprotein B-100
• Apolipoproteins B
• Blood Glucose
• Carbon Isotopes
• Fatty Acids, Nonesterified
• Lipoproteins, VLDL

Topics

• Adipose Tissue
• Adult
• Apolipoprotein B-100
• Apolipoproteins B (blood, metabolism)
• Blood Glucose (analysis)
• Body Composition
• Carbon Isotopes
• Diabetes Mellitus (physiopathology)
• Diet
• Electric Impedance
• Fatty Acids, Nonesterified (blood)
• Gas Chromatography-Mass Spectrometry
• Glucose Intolerance (physiopathology)
• Homeostasis
• Humans
• Insulin Resistance
• Kinetics
• Lipoproteins, VLDL (metabolism)
• Liver (metabolism)
• Magnetic Resonance Imaging
• Male
• Middle Aged
• Obesity (physiopathology)
• Regression Analysis