Synergism between rViscumin and cisplatin is not dependent on ERCC-1 expression.

Abstract	Interactions between recombinant mistletoe lectin (rViscumin) and anticancer drugs were investigated in vitro. rViscumin enhanced the cytotoxic effects of vincristine, mafosfamide, idarubicin and cisplatin in the human leukemia cell lines K562 and KG1a. In human marrow progenitor cells, rViscumin inhibited colony formation and did not exert any protective activity against cisplatin-induced inhibition of clonogenicity. Quantitative real-time reverse transcription polymerase chain reaction analysis revealed that cisplatin treatment of K562 cells resulted in a 1.9-fold increase in mRNA expression of the nucleotide excision repair gene ERCC-1. This upregulation was not prevented when cells were post-incubated with rViscumin. Our study provides evidence that rViscumin is capable of enhancing cytotoxicity of anticancer agents in vitro. This synergism appears to be independent of transcriptional activity of DNA repair relevant genes.
Authors	Oliver Galm, Ursula Fabry, Thomas Efferth, Rainhardt Osieka
Journal	Cancer letters (Cancer Lett) Vol. 187 Issue 1-2 Pg. 143-51 (Dec 10 2002) ISSN: 0304-3835 [Print] Ireland
PMID	12359362 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Adjuvants, Immunologic Antineoplastic Agents DNA-Binding Proteins Plant Preparations Plant Proteins Proteins RNA, Messenger RNA, Neoplasm Ribosome Inactivating Proteins, Type 2 Toxins, Biological ribosome inactivating protein, Viscum ERCC1 protein, human Endonucleases Cisplatin
Topics	Adjuvants, Immunologic (pharmacology) Antineoplastic Agents (pharmacology) Base Pair Mismatch Bone Marrow (drug effects, metabolism) Cell Division (drug effects) Cisplatin (pharmacology) DNA Repair DNA-Binding Proteins Drug Synergism Endonucleases Hematopoietic Stem Cells (drug effects, metabolism) Humans Plant Preparations (pharmacology) Plant Proteins Proteins (genetics, metabolism) RNA, Messenger (metabolism) RNA, Neoplasm Reverse Transcriptase Polymerase Chain Reaction Ribosome Inactivating Proteins, Type 2 Toxins, Biological (pharmacology) Tumor Cells, Cultured (drug effects, metabolism) Up-Regulation

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