Borrelia burgdorferi-specific monoclonal antibodies derived from mice primed with Lyme disease spirochete-infected Ixodes scapularis ticks.

Abstract

We have generated a panel of IgG monoclonal antibodies (MAbs) directed against Borrelia burgdorferi strain B31 antigens, using a method whereby mice were primed with organisms naturally inoculated by Ixodes scapularis nymphal ticks. Western blot analysis showed that these MAbs recognized several B. burgdorferi B31 antigens, including the complement inhibitor factor H-binding proteins ErpA/I/N and ErpC. Two other MAbs were specific for the RevA protein, and have enabled characterization of that previously unknown protein. The data presented here suggest that the production of MAbs from animals infected by tick-bite is a potentially useful tool for the identification of novel proteins synthesized by B. burgdorferi during mammalian infection.

• Authors: M Lamine Mbow, Robert D Gilmore Jr, Brian Stevenson, William T Golde, Joseph Piesman, Barbara J B Johnson
• Journal: Hybridoma and hybridomics (Hybrid Hybridomics) Vol. 21 Issue 3 Pg. 179-82 (Jun 2002) ISSN: 1536-8599 [Print] United States
• PMID: 12165143 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Bacterial Proteins

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antibody Specificity
• Bacterial Proteins (immunology)
• Blotting, Western
• Borrelia burgdorferi (immunology)
• Female
• Hot Temperature
• Immunization
• Ixodes (immunology)
• Lyme Disease (immunology)
• Lymph Nodes (immunology)
• Mice
• Mice, Inbred BALB C
• Spleen (immunology)
• Tick Infestations (immunology)