Effects of combinations of 7beta-hydroxycholesterol and anticancer drugs or ionizing radiation on the proliferation of cultured tumor cells.

Abstract

Oxysterols compose a large class of natural substances endowed in a number of cases with marked antiproliferative and cytotoxic activities. The consequences of treatments combining 7beta-hydroxycholesterol (7beta-OHC) or XG-142 (a galactose-linked hydrosoluble derivative of 7beta-OHC) with drugs used in cancer chemotherapy or gamma radiation has been evaluated upon a variety of tumor cell lines: Hep-G2, U937, K562 cells, its adriamycin-resistant variant K562 Adr+ and RDM4. Proliferation was assessed by the Uptiblue assay and the [3H]Thymidine incorporation test. Results indicated that 7beta-OHC increased the sensitivity of tumor cells to adriamycin, VP-16, 5-FU and bleomycin to various degrees. 7beta-OHC was found to reinforce the susceptibility of K562 adriamycin-resistant cells to this drug. In RDM4 cells, an enhanced radiosensitivity by 7beta-OHC was also obtained, whereas XG-142 was less efficient in provoking such an effect.

• Authors: Jin Won Hyun, Vincent Holl, Denis Weltin, Patrick Dufour, Bang Luu, Pierre Bischoff
• Journal: Anticancer research (Anticancer Res) 2002 Mar-Apr Vol. 22 Issue 2A Pg. 943-8 ISSN: 0250-7005 [Print] Greece
• PMID: 12014676 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydroxycholesterols
• Radiation-Sensitizing Agents
• Bleomycin
• cholest-5-en-3 beta,7 alpha-diol
• Doxorubicin
• Fluorouracil

Topics

• Antineoplastic Combined Chemotherapy Protocols (pharmacology)
• Bleomycin (administration & dosage)
• Cell Division (drug effects, radiation effects)
• Cell Survival (drug effects, radiation effects)
• Combined Modality Therapy
• Doxorubicin (administration & dosage)
• Drug Synergism
• Fluorouracil (administration & dosage)
• Humans
• Hydroxycholesterols (administration & dosage, pharmacology)
• Radiation-Sensitizing Agents (pharmacology)
• Tumor Cells, Cultured (drug effects, radiation effects)