Abstract |
Volatile anesthetics stimulate, but hyperglycemia attenuates, the activity of mitochondrial ATP-regulated K(+) channels. We tested the hypothesis that diabetes mellitus interferes with isoflurane-induced preconditioning. Acutely instrumented, barbiturate-anesthetized dogs were randomly assigned to receive 0, 0.32, or 0.64% end-tidal concentrations of isoflurane in the absence or presence of diabetes (3 wk after administration of alloxan and streptozotocin) in six experimental groups. All dogs were subjected to a 60-min left anterior descending coronary artery occlusion followed by 3 h of reperfusion. Myocardial infarct size ( triphenyltetrazolium staining) was 29 +/- 3% (n = 8) of the left ventricular area at risk in control experiments. Isoflurane reduced infarct size (15 +/- 2 and 13 +/- 1% during 0.32 and 0.64% concentrations; n = 8 and 7 dogs, respectively). Diabetes alone did not alter infarct size (30 +/- 3%; n = 8) but blocked the protective effects of 0.32% (27 +/- 2%; n = 7) and not 0.64% isoflurane (18 +/- 3%; n = 7). Infarct size was directly related to blood glucose concentrations in diabetic dogs, but this relationship was abolished by higher concentrations of isoflurane. The results indicate that blood glucose and end-tidal isoflurane concentrations are important determinants of infarct size during anesthetic-induced preconditioning.
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Authors | Katsuya Tanaka, Franz Kehl, Weidong Gu, John G Krolikowski, Paul S Pagel, David C Warltier, Judy R Kersten |
Journal | American journal of physiology. Heart and circulatory physiology
(Am J Physiol Heart Circ Physiol)
Vol. 282
Issue 6
Pg. H2018-23
(Jun 2002)
ISSN: 0363-6135 [Print] United States |
PMID | 12003806
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Anesthetics, Inhalation
- Blood Glucose
- Isoflurane
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Topics |
- Anesthetics, Inhalation
(administration & dosage, therapeutic use)
- Animals
- Blood Glucose
(analysis)
- Constriction
- Coronary Vessels
- Diabetes Mellitus, Experimental
(complications)
- Dogs
- Female
- Ischemic Preconditioning
(methods)
- Isoflurane
(administration & dosage, therapeutic use)
- Male
- Myocardial Infarction
(pathology)
- Myocardial Ischemia
(physiopathology)
- Myocardial Reperfusion Injury
(complications, pathology, prevention & control)
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