Premature aging in mice deficient in DNA repair and transcription.

Abstract

One of the factors postulated to drive the aging process is the accumulation of DNA damage. Here, we provide strong support for this hypothesis by describing studies of mice with a mutation in XPD, a gene encoding a DNA helicase that functions in both repair and transcription and that is mutated in the human disorder trichothiodystrophy (TTD). TTD mice were found to exhibit many symptoms of premature aging, including osteoporosis and kyphosis, osteosclerosis, early greying, cachexia, infertility, and reduced life-span. TTD mice carrying an additional mutation in XPA, which enhances the DNA repair defect, showed a greatly accelerated aging phenotype, which correlated with an increased cellular sensitivity to oxidative DNA damage. We hypothesize that aging in TTD mice is caused by unrepaired DNA damage that compromises transcription, leading to functional inactivation of critical genes and enhanced apoptosis.

• Authors: Jan de Boer, Jaan Olle Andressoo, Jan de Wit, Jan Huijmans, Rudolph B Beems, Harry van Steeg, Geert Weeda, Gijsbertus T J van der Horst, Wibeke van Leeuwen, Axel P N Themmen, Morteza Meradji, Jan H J Hoeijmakers
• Journal: Science (New York, N.Y.) (Science) Vol. 296 Issue 5571 Pg. 1276-9 (May 17 2002) ISSN: 1095-9203 [Electronic] United States
• PMID: 11950998 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA-Binding Proteins
• Proteins
• RNA-Binding Proteins
• Transcription Factors
• Xeroderma Pigmentosum Group A Protein
• Xpa protein, mouse
• DNA Helicases
• Xeroderma Pigmentosum Group D Protein
• Ercc2 protein, mouse

Topics

• Aging
• Aging, Premature (etiology)
• Animals
• Apoptosis
• Bone Density
• Cachexia (etiology)
• Crosses, Genetic
• DNA Damage
• DNA Helicases (genetics, physiology)
• DNA Repair
• DNA-Binding Proteins (genetics, physiology)
• Female
• Fertility
• Gene Targeting
• Growth Disorders (etiology, genetics)
• Hair Diseases (genetics)
• Kyphosis (etiology, genetics, pathology)
• Male
• Mice
• Mutation
• Oxidative Stress
• Phenotype
• Point Mutation
• Proteins (genetics, physiology)
• RNA-Binding Proteins (genetics, physiology)
• Transcription Factors
• Transcription, Genetic
• Xeroderma Pigmentosum Group A Protein
• Xeroderma Pigmentosum Group D Protein