Identification of type II collagen peptide 261-273-specific T cell clones in a patient with relapsing polychondritis.

Abstract

OBJECTIVE:

To characterize and clone T cells specific for type II collagen (CII) in a patient with relapsing polychondritis (RP) and to establish whether the immunodominant epitope of CII determined in HLA transgenic mice is used in the human autoimmune response to CII.

METHODS:

T cell responses to CII were examined in a patient with RP, who was heterozygous for the HLA-DR allele DRB1*0101/DRB1*0401. T cell clones were established from this patient and characterized for peptide specificity, class II restriction, cytokine production, and staining with HLA-DRB1*0401 class II tetramers.

RESULTS:

A response to CII and the peptide 255-273 was present in this patient. T cells specific for the CII epitope 261-273 were cloned. Evaluation of these clones demonstrated a response to CII 261-273 in the context of both DR alleles. HLA-DR4 CII tetramer did not demonstrate staining of either CII-specific DRB1*0401-restricted T cell clones or a polyclonal population of CII-reactive T cells from this individual.

CONCLUSION:

T cells directed against CII were present in this patient with RP. Also, T cell clones isolated from this individual were found to be specific for the CII peptide 261-273 and were restricted to either the DRB1*0101 or the DRB1*0401 allele. These findings establish that a T cell response directed against CII is present in this patient with RP and that the CII peptide 261-273 plays a role in the human immune response to CII.

• Authors: Jane H Buckner, Megan Van Landeghen, William W Kwok, Laura Tsarknaridis
• Journal: Arthritis and rheumatism (Arthritis Rheum) Vol. 46 Issue 1 Pg. 238-44 (Jan 2002) ISSN: 0004-3591 [Print] United States
• PMID: 11817597 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Collagen Type II
• Epitopes
• HLA-DR Antigens
• HLA-DRB1 Chains
• HLA-DRB1*04:01 antigen
• Peptide Fragments

Topics

• Adult
• Clone Cells (immunology)
• Collagen Type II (analysis, immunology)
• Epitopes
• Flow Cytometry
• HLA-DR Antigens (immunology)
• HLA-DRB1 Chains
• Histocompatibility Testing
• Humans
• Male
• Peptide Fragments (immunology)
• Polychondritis, Relapsing (immunology)
• Staining and Labeling
• T-Lymphocytes (chemistry, cytology, immunology)