Erysipelothrix rhusiopathiae YS-1 as a live vaccine vehicle for heterologous protein expression and intranasal immunization of pigs.

Abstract

We have developed a system in which a foreign antigen is delivered and expressed on the surface of an attenuated strain of Erysipelothrix rhusiopathiae YS-1 and have examined the ability of a such recombinant E. rhusiopathiae strain to function as a mucosal vaccine vector. The C-terminal portion, including two repeat regions, R1 and R2, of the P97 adhesin of Mycoplasma hyopneumoniae strain E-1 was successfully translocated and expressed on the E. rhusiopathiae YS-1 cell surface after it was fused to SpaA.1, a cell surface protective antigen of E. rhusiopathiae. BALB/c mice subcutaneously immunized with the E. rhusiopathiae recombinant strains developed specific antibodies against SpaA.1 protein and were protected from lethal challenge with the highly virulent homologous E. rhusiopathiae Fujisawa-SmR strain, showing the efficacy of this heterologous-antigen expression system as a vaccine against E. rhusiopathiae infection. To determine whether protective immune responses are induced in target species, newborn, specific-pathogen-free piglets were immunized intranasally with a recombinant strain designated YS-19. The immunized piglets developed specific anti-SpaA.1 immunoglobulin G (IgG) antibodies in their serum and were protected from death by erysipelas, showing that mucosal vaccination of piglets with YS-19 induces systemic immune responses. Furthermore, YS-19-immunized piglets showed higher levels of P97-specific IgA antibodies in the respiratory tract than did YS-1-immunized piglets. Thus, E. rhusiopathiae YS-1 appears to be a promising vaccine vector for mucosal delivery that can induce local and systemic immune responses.

• Authors: Yoshihiro Shimoji, Eiji Oishi, Takashi Kitajima, Yoshihiro Muneta, Shinya Shimizu, Yasuyuki Mori
• Journal: Infection and immunity (Infect Immun) Vol. 70 Issue 1 Pg. 226-32 (Jan 2002) ISSN: 0019-9567 [Print] United States
• PMID: 11748187 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adhesins, Bacterial
• Antibodies, Bacterial
• Bacterial Vaccines
• Immunoglobulin A
• P97 protein, Mycoplasma
• Vaccines, Attenuated
• Vaccines, Synthetic

Topics

• Adhesins, Bacterial (genetics, immunology)
• Administration, Intranasal
• Animals
• Antibodies, Bacterial (immunology)
• Bacterial Vaccines (genetics, immunology)
• Cell Membrane (metabolism)
• Erysipelothrix (genetics, immunology, pathogenicity)
• Erysipelothrix Infections (prevention & control)
• Female
• Gene Expression
• Genetic Vectors (genetics, immunology)
• Immunoglobulin A (immunology)
• Mice
• Mice, Inbred BALB C
• Recombination, Genetic
• Respiratory System (immunology)
• Swine
• Vaccination
• Vaccines, Attenuated
• Vaccines, Synthetic (genetics, immunology)
• Virulence