Alanyl-glutamine enriched total parenteral nutrition improves local, systemic, and remote organ responses to intraperitoneal bacterial challenge.

Abstract

BACKGROUND:

Standard total parenteral nutrition (STD-TPN) may diminish host defense against infection. Glutamine (Gln) is suggested to enhance host immunity. This study investigated the effects of antecedent alanyl-glutamine enriched TPN (Ala-Gln-TPN) on host responses to intraperitoneal bacterial challenge compared with STD-TPN.

METHODS:

Rats were divided into STD-TPN and Ala-Gln-TPN groups. They received isocaloric and isonitrogenous nutrition for 7 days and were challenged intraperitoneally with E. coli. Rats were killed before (0 hour) challenge and at 2 and 6 hours after challenge. Bacterial numbers in peritoneal lavage fluid (PLF), liver, spleen, and blood were determined. Tumor necrosis factor-alpha (TNF), interleukin (IL)-8, and interferon-gamma (IFN) in plasma and PLF were measured. Hepatic TNF, splenic TNF, and splenic IFN levels were determined.

RESULTS:

The numbers of E. coli in systemic blood at 2 hours after intraperitoneal bacterial challenge were significantly lower in the Ala-Gln-TPN than in STD-TPN group. E. coli numbers in blood significantly correlated with those in the liver. The Ala-Gln-TPN also resulted in significantly higher PLF and hepatic TNF levels, higher splenic IFN levels, and lower plasma IL-8 levels at 6 hours after challenge compared with the STD-TPN.

CONCLUSIONS:

Antecedent Ala-Gln enriched TPN enhance local, systemic, and remote organ immune responses to intraperitoneal bacterial challenge. Ala-Gln-TPN may enhance host defense and be more beneficial than standard TPN in sepsis.

• Authors: M T Lin, H Saito, S Furukawa, R Fukushima, F Kazuhiko, P H Lee, K J Chang, W J Chen
• Journal: JPEN. Journal of parenteral and enteral nutrition (JPEN J Parenter Enteral Nutr) 2001 Nov-Dec Vol. 25 Issue 6 Pg. 346-51 ISSN: 0148-6071 [Print] United States
• PMID: 11688940 (Publication Type: Journal Article)

Chemical References

• Dipeptides
• Interleukin-8
• Tumor Necrosis Factor-alpha
• Interferon-gamma
• alanylglutamine

Topics

• Animals
• Ascitic Fluid (cytology, immunology, microbiology)
• Bacteremia (immunology)
• Dipeptides (administration & dosage)
• Escherichia coli (growth & development, immunology)
• Escherichia coli Infections (immunology, therapy)
• Injections, Intraperitoneal
• Interferon-gamma (analysis)
• Interleukin-8 (analysis)
• Liver (immunology, microbiology)
• Lung (cytology, immunology)
• Male
• Parenteral Nutrition, Total
• Peritoneal Lavage
• Random Allocation
• Rats
• Rats, Wistar
• Spleen (immunology, microbiology)
• Tumor Necrosis Factor-alpha (analysis)