Abstract | BACKGROUND: METHODS: GA, a recognized inhibitor of 11beta-HSD2 was supplemented to the drinking water (3 g/l) of Wistar Kyoto rats over a period of 21 days. From day 8 to 21, spironolactone (5.8+/-0.6 mg/kg/day), darusentan (45.2+/-6.5 mg/kg/day), or placebo was added to chow (n=7 per group). After the animals were killed, vascular function of isolated renal artery segments was assessed by isometric tension recording. RESULTS: Relaxation of pre-constricted renal artery segments in response to acetylcholine (10(-10) to 10(-5) mol/l) was impaired by GA as compared with controls (12+/-4% vs 98+/-5% of norepinephrine 3x10(-7) mol/l), whereas endothelium independent relaxations were unaffected. Endothelin receptor antagonism improved renovascular endothelium-dependent relaxation (32+/-4%, P<0.05 vs placebo) whereas endothelium-dependent relaxation was completely normalized by aldosterone receptor antagonism (85+/-4%, P<0.01 vs placebo). CONCLUSIONS:
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Authors | T Quaschning, F Ruschitzka, B Niggli, C M Lunt, S Shaw, M Christ, M Wehling, T F Lüscher |
Journal | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
(Nephrol Dial Transplant)
Vol. 16
Issue 11
Pg. 2146-51
(Nov 2001)
ISSN: 0931-0509 [Print] England |
PMID | 11682659
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Endothelin Receptor Antagonists
- Mineralocorticoid Receptor Antagonists
- Vasoconstrictor Agents
- Spironolactone
- Potassium Chloride
- Glycyrrhizic Acid
- Norepinephrine
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Topics |
- Animals
- Blood Vessels
(drug effects, physiopathology)
- Endothelin Receptor Antagonists
- Endothelium, Vascular
(physiopathology)
- Glycyrrhiza
(adverse effects)
- Glycyrrhizic Acid
(pharmacology)
- Hypertension
(etiology, physiopathology)
- Male
- Mineralocorticoid Receptor Antagonists
- Norepinephrine
(pharmacology)
- Potassium Chloride
(pharmacology)
- Rats
- Rats, Inbred WKY
- Renal Artery
(drug effects)
- Renal Circulation
(drug effects)
- Spironolactone
(pharmacology)
- Vasoconstriction
(drug effects)
- Vasoconstrictor Agents
(pharmacology)
- Vasodilation
(drug effects)
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