Neutralization of a snake venom hemorrhagic metalloproteinase prevents coagulopathy after subcutaneous injection of Bothrops jararaca venom in rats.

Abstract

Coagulopathy is one of the major complications following envenomations by crotalid and viperid snakes. The present study was undertaken to examine the effect of a hemorrhagic metalloproteinase in Bothrops jararaca venom, jararafibrase I (JF I), on the development of coagulopathy using rat snakebite model. Coagulation parameters were monitored after subcutaneous injection of B. jararaca crude venom, JF I-neutralized venom and purified JF I in rats. Crude venom induced unclottable blood and fibrinogen consumption, while JF I-neutralized venom and purified JF I did not induce coagulopathy. Plasma venom antigen level of rats given JF I-neutralized venom was lower than that of rats given crude venom. We conclude that venom hemorrhagic metalloproteinases play an important role in the development of coagulopathy through rapid spreading of venom coagulation components from the injected area into systemic circulation.

• Authors: Keita Anai, Masahiko Sugiki, Etsuo Yoshida, Masugi Maruyama
• Journal: Toxicon : official journal of the International Society on Toxinology (Toxicon) Vol. 40 Issue 1 Pg. 63-8 (Jan 2002) ISSN: 0041-0101 [Print] England
• PMID: 11602280 (Publication Type: Journal Article)

Chemical References

• Antivenins
• Crotalid Venoms
• Fibrinolytic Agents
• Immunoglobulin G
• Metalloendopeptidases
• jararafibrase I

Topics

• Animals
• Antivenins (pharmacology, therapeutic use)
• Blood Coagulation (drug effects)
• Blotting, Western
• Bothrops
• Crotalid Venoms (antagonists & inhibitors, enzymology, immunology)
• Disease Models, Animal
• Electrophoresis, Polyacrylamide Gel
• Fibrinolytic Agents
• Hemorrhage (prevention & control, therapy)
• Immunoglobulin G (therapeutic use)
• Male
• Metalloendopeptidases (antagonists & inhibitors, immunology)
• Prothrombin Time
• Rabbits
• Rats
• Rats, Wistar