Abstract | BACKGROUND: METHODS: The authors investigated the expression of survivin in 4 pancreatic carcinoma cell lines and 56 human pancreatic tissues (5 cases of normal, 12 cases of chronic pancreatitis [CP], 26 cases of pancreatic duct cell adenocarcinoma [PDC], 16 lesions of 13 intraductal papillary-mucinous tumor [IPMT] by immunohistochemistry, immunoblotting, and reverse transcription-polymerase chain reaction (RT-PCR) to examine the association of its expression with tumor apoptosis and/or tumorigenesis. RESULTS:
Survivin expression was found in the tumor cells but not in the nonneoplastic pancreatic tissues (normal and CP tissues). Survivin expression was observed in 20 of 26 cases of PDC (76.9%) and in 9 of 16 IPMT lesions that ranged from adenoma to invasive (56.3%) by immunohistochemistry. Survivin was more frequently expressed in malignant tumors than in benign tumors (P = 0.0089). In PDC, high levels of survivin expression were associated significantly with a reduction in the apoptotic index of the tumor cells (0.445% +/- 0.150% vs. 0.961% +/- 0.378%; P < 0.0001). CONCLUSIONS: These data suggest that the expression of survivin may be upregulated during an early step of tumorigenesis and during the development of cancer by reducing the cancer cell apoptosis.
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Authors | K Satoh, K Kaneko, M Hirota, A Masamune, A Satoh, T Shimosegawa |
Journal | Cancer
(Cancer)
Vol. 92
Issue 2
Pg. 271-8
(Jul 15 2001)
ISSN: 0008-543X [Print] United States |
PMID | 11466679
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2001 American Cancer Society. |
Chemical References |
- BIRC5 protein, human
- Inhibitor of Apoptosis Proteins
- Microtubule-Associated Proteins
- Neoplasm Proteins
- Survivin
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Topics |
- Adenocarcinoma
(pathology)
- Apoptosis
- Carcinoma, Medullary
(pathology)
- Carcinoma, Pancreatic Ductal
(pathology)
- Carcinoma, Papillary
(pathology)
- Cell Transformation, Neoplastic
- Chronic Disease
- Humans
- Immunoblotting
- Immunohistochemistry
- Inhibitor of Apoptosis Proteins
- Microtubule-Associated Proteins
- Neoplasm Proteins
- Pancreas
(physiology)
- Pancreatic Neoplasms
(pathology)
- Pancreatitis
(pathology)
- Protein Biosynthesis
- Reverse Transcriptase Polymerase Chain Reaction
- Survivin
- Tumor Cells, Cultured
(physiology)
- Up-Regulation
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