Abstract |
We have isolated a monoclonal antibody, clone betaE11, which recognizes an antigen that is highly abundant on the surface of mitotic vascular endothelial cells and tumor cells. By sodium dodecyl sulfate- polyacrylamide gel electrophoresis and Western blotting, expression of this 190-kd antigen is approximately threefold higher in mitotic versus interphase endothelial cells. Treatment of tumor cells with an antibody to the betaE11 antigen inhibits their growth in a dose-dependent manner in vitro with maximal inhibition at an antibody concentration of 1 microg/ml. Different tumor cell lines demonstrate varying sensitivities to anti-betaE11 with the following order of growth inhibition: colon > prostate = glioma > melanoma = fibroblast > breast > liver. Furthermore, the betaE11 antigen localizes to regions of prostatic intraductal neoplasia in paraffin-embedded sections. Mass spectrometry of the cell-derived betaE11 protein and V8-protease fingerprint analysis indicate that the betaE11 antigen is nearly identical to the 4F2 heavy chain antigen, a cell surface protein that has been implicated in cell activation and proliferation. Expression of the betaE11 antigen during mitosis functionally links it to a fundamental aspect of cell proliferation, and its spatial localization on the surface of both proliferating endothelium and tumor cells demonstrates its potential for tumor immunotherapy.
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Authors | M Papetti, I M Herman |
Journal | The American journal of pathology
(Am J Pathol)
Vol. 159
Issue 1
Pg. 165-78
(Jul 2001)
ISSN: 0002-9440 [Print] United States |
PMID | 11438464
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies
- Antigens, CD
- Antigens, Surface
- Carrier Proteins
- Fusion Regulatory Protein-1
- betaE11 antigen, bovine
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Topics |
- Animals
- Antibodies
(pharmacology)
- Antigens, CD
(chemistry, physiology)
- Antigens, Surface
(chemistry, immunology, metabolism)
- Blotting, Western
- Carrier Proteins
(chemistry, physiology)
- Cattle
- Cell Division
(drug effects, physiology)
- Cells, Cultured
- Endothelium, Vascular
(cytology, metabolism)
- Fusion Regulatory Protein-1
- Glycosylation
- Mitosis
(physiology)
- Neoplasms
(metabolism, pathology)
- Protein Processing, Post-Translational
- Tissue Distribution
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