Expression of epithelial neutrophil-activating peptide 78 in cultured human endometrial stromal cells.

Abstract

It has been demonstrated that human endometrial stromal cells (ESC) produce a variety of chemokines in vivo and in vitro. To evaluate the expression of epithelial neutrophil-activating peptide 78 (ENA-78) in the endometrium, concentrations of ENA-78 in cyclic endometrial tissues were measured using enzyme-linked immunosorbent assay. The expression of ENA-78 was also detected in cyclic endometrium by immunohistochemistry. Endometrial tissues in the secretory phase contained higher amounts of ENA-78 protein than did those in the proliferative phase. Immunofluorescence staining revealed that ENA-78 protein was localized mainly in the stroma of endometrium. In addition, to evaluate the involvement of inflammatory mediators and ovarian steroid hormones in the production of ENA-78 by ESC was evaluated by in-vitro studies. Unstimulated ESC constitutively secreted ENA-78. Progesterone, lipopolysaccharide, tumour necrosis factor-alpha, and interleukin-1beta significantly stimulated the expression of ENA-78 by ESC. It is suggested that the production of ENA-78 by ESC is regulated by progesterone as well as by the inflammatory mediators. The modulation of ENA-78 concentration in the local environment by these mediators may contribute to the normal and pathological processes of human reproduction through regulation of leukocyte trafficking into the endometrium.

• Authors: K Nasu, K Arima, K Kai, K Fujisawa, M Nishida, I Miyakawa
• Journal: Molecular human reproduction (Mol Hum Reprod) Vol. 7 Issue 5 Pg. 453-8 (May 2001) ISSN: 1360-9947 [Print] England
• PMID: 11331668 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CXCL5 protein, human
• Chemokine CXCL5
• Chemokines, CXC
• Culture Media
• Interleukin-1
• Interleukin-8
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha

Topics

• Cells, Cultured
• Chemokine CXCL5
• Chemokines, CXC
• Culture Media
• Endometrium (cytology, metabolism)
• Female
• Humans
• Interleukin-1 (pharmacology)
• Interleukin-8 (analogs & derivatives, biosynthesis)
• Lipopolysaccharides (pharmacology)
• Stromal Cells (cytology, drug effects, metabolism)
• Tumor Necrosis Factor-alpha (pharmacology)