Abstract |
A T helper (Th)1/Th2 model has been applied to as a system regulating the cytokine network during pregnancy. To evaluate the effects of interleukin (IL)-4, a Th2 cytokine, on the cytokine production by endometrial stromal cells (ESC), an enzyme-linked immunosorbent assay was used to measure the concentrations of IL-6, IL-8, monocyte chemoattractant protein-1 (MCP-1), and macrophage colony-stimulating factor ( M-CSF) in the culture media of ESC and of an endometrial stromal sarcoma cell line, MaMi, following the addition of recombinant human IL-4. The expression of mRNAs for IL-6 and IL-8 in ESC after stimulation with IL-4 was also evaluated by Northern blot analysis. Increases in the concentrations of IL-6, IL-8, MCP-1, and M-CSF in the culture media of ESC and MaMi cells were observed on the addition of increasing amounts of IL-4. This cytokine also stimulated the transcription of IL-6 and IL-8 in ESC in a dose-dependent manner. It is suggested that IL-4 secreted by the maternal decidual tissue as well as by the developing embryo may stimulate the production of IL-6, IL-8, MCP-1, and M-CSF by ESC. The increased concentration of these cytokines in the local environment may contribute to the maintenance of early pregnancy by modulating the immune reaction at the feto-maternal interface.
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Authors | K Nasu, T Sugano, K Fujisawa, K Arima, H Narahara, I Miyakawa |
Journal | Molecular human reproduction
(Mol Hum Reprod)
Vol. 7
Issue 3
Pg. 265-70
(Mar 2001)
ISSN: 1360-9947 [Print] England |
PMID | 11228246
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemokine CCL2
- Culture Media
- Interleukin-6
- Interleukin-8
- RNA, Messenger
- Interleukin-4
- Macrophage Colony-Stimulating Factor
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Topics |
- Animals
- Chemokine CCL2
(biosynthesis)
- Culture Media
- Endometrium
(cytology)
- Female
- Gene Expression
- Humans
- Interleukin-4
(immunology, pharmacology)
- Interleukin-6
(biosynthesis, genetics)
- Interleukin-8
(biosynthesis, genetics)
- Macrophage Colony-Stimulating Factor
(biosynthesis)
- RNA, Messenger
- Stromal Cells
(drug effects, immunology)
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