Molecular cloning of human and mouse DJ-1 genes and identification of Sp1-dependent activation of the human DJ-1 promoter.

Abstract

DJ-1 has been identified as a novel oncogene that transforms mouse NIH3T3 cells in cooperation with activated ras. Subsequently, two other groups have identified SP22 or CAP-1, rat homologs of human DJ-1, as a sperm protein targeted by some toxicants leading to male infertility, and another group has also reported that RS, the same as human DJ-1, is a component of an RNA-binding protein complex. To characterize the expression or functional importance of DJ-1, the genomic DNAs of both human and mouse DJ-1 were cloned and characterized. Both genomic DNAs comprise 7 exons spanning about 16-24 kb, in which 2-6 exons encode the DJ-1 protein. The human DJ-1 gene was mapped at chromosome 1p36.2-p36.3, a region that has been shown to be a hot spot of chromosome abnormalities in several tumor cells. To analyze the promoter of the human DJ-1 gene, a series of deletion constructs of the region upstream of exon 2 were linked to the luciferase gene, and their luciferase activities were measured in human HeLa cells. Of the many putative transcription regulatory sequences, the Sp1 site present at -100 from the transcription initiation site contributed to the major promoter activity, and Sp1 was identified as a protein binding to this site by a mobility shift assay using HeLa nuclear extract.

• Authors: T Taira, K Takahashi, R Kitagawa, S M Iguchi-Ariga, H Ariga
• Journal: Gene (Gene) Vol. 263 Issue 1-2 Pg. 285-92 (Jan 24 2001) ISSN: 0378-1119 [Print] Netherlands
• PMID: 11223268 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• Intracellular Signaling Peptides and Proteins
• Oncogene Proteins
• Recombinant Fusion Proteins
• Sp1 Transcription Factor
• DNA
• Luciferases
• PARK7 protein, human
• Protein Deglycase DJ-1

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Cell Line
• Chromosome Mapping
• Chromosomes, Human, Pair 1 (genetics)
• Cloning, Molecular
• DNA (chemistry, genetics, metabolism)
• DNA, Complementary (chemistry, genetics)
• Exons
• Genes (genetics)
• HeLa Cells
• Humans
• Intracellular Signaling Peptides and Proteins
• Introns
• K562 Cells
• Luciferases (genetics, metabolism)
• Mice
• Molecular Sequence Data
• Oncogene Proteins (genetics, metabolism)
• Promoter Regions, Genetic (genetics)
• Protein Binding
• Protein Deglycase DJ-1
• Recombinant Fusion Proteins (genetics, metabolism)
• Sequence Alignment
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid
• Sp1 Transcription Factor (metabolism)
• Transcription, Genetic
• Tumor Cells, Cultured
• U937 Cells