Ascorbyl free radical release in diabetic patients.

Abstract

The objective of this study was to verify the presence of oxidative stress in type 1 and type 2 diabetic patients and to provide evidences for an use of ascorbyl free radical (AFR)/vitamin C ratios as tools exploring the level of oxidative stress in diabetic patients. TBARS levels, oxygen-radical absorbing capacity assay and AFR release assessed by electron paramagnetic resonance (EPR) were used to explore the existence of oxidative stress in diabetes. Endogenous antioxidants (alpha-tocopherol, vitamin C and uric acid) were also measured. Scavenging capacities of plasma were decreased in diabetic patients. A significant decrease of plasma vitamin C and an increase of AFR/vitamin C ratios were noted in type 2 diabetic patients. Uricemia was decreased in type 1 diabetic patients. Our results suggest a possible use of AFR/vitamin C ratios as indicators of oxidative stress in diabetes mellitus.

• Authors: C Courderot-Masuyer, J J Lahet, B Verges, J M Brun, L Rochette
• Journal: Cellular and molecular biology (Noisy-le-Grand, France) (Cell Mol Biol (Noisy-le-grand)) Vol. 46 Issue 8 Pg. 1397-401 (Dec 2000) ISSN: 0145-5680 [Print] France
• PMID: 11156484 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Cholesterol, HDL
• Cholesterol, LDL
• Free Radicals
• Thiobarbituric Acid Reactive Substances
• Triglycerides
• Vitamin E
• Uric Acid
• Cholesterol
• Ascorbic Acid

Topics

• Antioxidants (metabolism)
• Ascorbic Acid (blood)
• Cholesterol (blood)
• Cholesterol, HDL (blood)
• Cholesterol, LDL (blood)
• Diabetes Mellitus, Type 1 (blood)
• Diabetes Mellitus, Type 2 (blood)
• Electron Spin Resonance Spectroscopy
• Female
• Free Radicals (blood)
• Humans
• Lipid Peroxidation
• Male
• Oxidative Stress
• Spectrophotometry
• Thiobarbituric Acid Reactive Substances
• Triglycerides (blood)
• Uric Acid (blood)
• Vitamin E (blood)